JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effect of FLIP-siRNA-mediated gene silencing on bionomics and growth inhibition in the ovarian cancer cell line A2780

WANG Yu1，LIU Pei-shu1，MAO Hong-luan1，ZHOU Li-guang1，SUN Yu-lan2，JIANG Hong3，WANG Rong3   

  1. 1. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, China;2. Department of Obstetrics, Xintai People′s Hospital, Tai′an 271200, Shandong， China
  • Received:2007-11-03 Revised:1900-01-01 Online:2008-02-16 Published:2008-02-16
  • Contact: LIU Pei-shu

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Abstract: To investigate the effects of FLIP-siRNA-mediated gene silencing on bionomics and growth inhibition in the ovarian cancer cell line A2780. Methods Based on the sequence of FLIP mRNA, three siRNAs were designed and chemically synthesized. They were transfected into the ovarian cancer cell line A2780 with LipofectamineTM2000. The FLIP mRNA and protein levels were determined by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blot. The FLIP-siRNA having the most powerful inhibition was selected. Cell growth inhibition and apoptosis were determined by MTT assay and flow cytometry. Results Short siRNAs targeting FLIP down-regulated the mRNA and the protein level of the FLIP oncogene（P＜0.01），　and the inhibition rate reached to 77.4％ and 66.1％, respectively. The proliferation of the ovarian cancer cell line A2780 was inhibited（P＜0.05）and the ratio of apoptosis significantly increased after transfection（P＜0.05）. Conclusion FLIP-siRNAs can significantly downregulate FLIP expression in transcriptional and translational levels, inhibit cell growth and induce cell apoptosis in the ovarian cancer cell line A2780．

Key words: RNA interference, Doublestranded RNA, Genes, FLIP, Ovarian neoplasms

CLC Number: 

  • R737.31
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