JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Apoptotic effects of ODC and AdoMetDC bi-antisense virus on esophageal cancer cell Eca109

XU Jie1, TIAN Hui1, LIU Xian-xi2, ZHANG Bing2, LI Wen-jun1, SONG Xu1   

  1. 1. Department of Thoracic Surgery, Qilu Hospital of Shandong University; 2. Experimental Center of Medical Molecular Biology, School of Medicine, Shandong University， Jinan 250012, China
  • Received:2008-04-09 Revised:1900-01-01 Online:2008-10-16 Published:2008-10-16
  • Contact: TIAN Hui

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Abstract: To explore the inhibitory effects of Ad-ODC-AdoMetDCas on polyamine biosynthesis and esophageal cancer cell apoptosis. Methods Adenovirus-mediated gene transduction efficiency was assessed by counting the GFP-positive cells by using MTT. The malignant phenotype of the Eca109 cells was assessed by a growth curve. Western Blot and HPLC were used to determine the ODC and AdoMetDC expressions and polyamine content in Eca109 cells. TUNEL was used to analyze cell apoptosis. Morphology of the apoptotic cells was observed by an electron microscope. ResultApproximately 70% of the Eca109 cells were infected with Ad-ODC-AdoMetDCas when MOI reached 50. The expression of ODC was inhibited in the infected tumor cells. Ad-ODC-AdoMetDCas could inhibit the Eca109 cell growth and invasive ability. TUNEL proved that Ad-ODC-AdoMetDCas could lead to cell apoptosis. Under an electron microscope, chromatin condensation, nuclear disintegration and formation of apoptotic bodies were found. Conclusion Ad-ODC-AdoMetDCas has significant inhibitory effects on esophageal cancer cell proliferation, leads to cell apoptosis and has therapeutic potential for the treatment of esophageal cancer.

Key words: Ornithine decarboxylase, S-adenosylmethionine decarboxylase, Polyamine, Esop neoplasms, Gene therapy

CLC Number: 

  • Q78
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