JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Proteomic analysis of renal cancer cells and kidney control tissues

JING Yong-sheng1，LIU Shi-lian1，ZHANG Xu-hua1，WU Xiao-qing2，YANG Yin-rong1,WANG Wei1   

  1. 1.Institute of Biochemistry and Molecular Biology；2.Tumor Center, Jinan Central Hospital,Shandong University
  • Received:2006-12-12 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
  • Contact: LIU Shi-lian

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Abstract: Objective： To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from renal cell carcinoma (RCC) tissues and paired tumoradjacent kidney tissues and to identify the differential expression proteins. Method： The total proteins of RCC tissues determined by histological and paired tumoradjacent kidney tissues were separated by two-dimensional gel electrophoresis(2-DE), analyzed by Image Master 2D software, and identified by peptide mass fingerprint(PMF) and database searching. Result: Well-resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry(MS) were matched in the SWISS-2DPAGE database． Conclusion: These data will be valuable for studying the diagnostic markers, mechanisms of metastasize and therapeutic targets.

Key words: Spectrum analysis, mass, Proteomics, Electrophoresis, gel, two-dimensional, Kidney neoplasms

CLC Number: 

  • R737.11
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