Abstract: Objective: To compare the oligonucleotide array and PNA array in determining mutation of HBV. Methods: Oligonucleotide and PNA probes which targeted 1762A-T and 1764G-A, 1858C-T and 1896G-A mutations and wild sites of HBV were designed and immobilized on aldehyde modified glass slides after synthesis. Target DNA was obtained by asymmetric PCR with a tamara-labeled upper primer. Result was produced by scanning and quantifying analysis after hybridization with labeled target DNA. Meanwhile, the PCR product was sequenced to analyze three mutation sites. Results: The ratio of fluorescence signals for 1762-1764 double site, 1858 and 1896 sites (wild type: mutation type) was 1.44, 1/1.24 and 1.05 in the oligonucleotide array and 2.8, 1/3.02, 1/1.71 in the PNA array. 1858T mutation and 1762A-1764G, 1896G wild sites were found in DNA sequencing. Conclusions: The oligonucleotide array and the PNA array could successfully detect the three mutation sites of HBV, which is in accordance with the result of DNA sequencing. PNA shows a high priority in attaching the target DNA and identifying the single-base mismatch.

Key words: Mutation, Hybridization, Oligonucleotide array, PNA array, Hepatitis B viruses