JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Survival and differentiation of GFP transgenic mice-derived neuroepithelial cells after being transplanted into rat brains

WANG Jia-zeng， BAO Li-hua， SUN Jin-hao， LI Zhen-hua， ZHANG Jing   

  1. Department of Anatomy， School of Medicine， Shandong University， Jinan 250012， Shandong, China
  • Received:2007-01-05 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
  • Contact: SUN Jin-hao

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Abstract: Objective: To observe the survival and differentiation of cells derived from the embryonic neural tube of green fluorescent protein (GFP) transgenic mice after being transplanted into the striatum of rats. Methods: Neuroepithelial stem cells derived from the embryonic neural tube of GFP transgenic mice were cultured and depurated in free serum medium containing B27, bFGF and EGF, and then they were directionally transplanted into the striatum of rats. Animals were sacrificed at different time points after being transplanted and frozen sectioned, and staining by MAP-2, GFAP, and TH was determined by immunohistochemical techniques. Results: Neuroepithelial stem cells derived from the embryonic neural tube could expand into neurospheres in the free serum medium and express nestin. The grafted regions were clearly visible, and the transplanted cells survived well and some of them differentiated into TH-positive cells. Conclusion: Embryonic neuroepithelial stem cells from GFP transgenic mice could be generated in vitro, survive and differentiate into neurons, especially dopaminergic neurons and neuroglia cells after being transplanted into rat brains.

Key words: Mice, transgenic, Neuroepithelium, Stem cells, Proliferation, Transplantation

CLC Number: 

  • R322.81
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