Abstract: Objective: To investigate the immune escape of hepatoma cell line HepG2.2.15 with low Fas expression as well as the effect of interferonα(IFN-α) on the apoptosis in heptoma cells through upregulating Fas expression. Methods: Flow cytometry was used to determine the Fas expression of the hepatoma cell line HepG2.2.15 and that treated with IFN-α. Resistance of hepatoma cells to Fas mediated apoptosis and the effect of IFN-α on the apoptosis were studied using antiFas agonistic monoclonal antibody CH11. Results: ①Fas expression of HepG2.2.15 was low and increased after the cells were treated with IFN-α; ②CH11 could not induce HepG2.2.15 cells to apoptosis. But the apoptosis induced by CH11 increased when the cells upregulated the Fas expression after treatment with IFN-α. Conclusions: The hepatoma cell line HepG2.2.15 could resist Fasmediated apoptosis. IFN-α is able to upregulate the Fas expression and subsequently promotes the CH11mediated apoptosis .

Key words: Liver neoplasms, Interferonalpha, Antigen, CD95, Cell apoptosis