Abstract: Objective： To investigate the levels of microvessel density(MVD), the expression of P-AKT and vascular endothelial growth factor(VEGF) in non-small cell lung cancer (NSCLC), and explore the correlation between them and the clinicopathological features of NSCLC. Methods: MVD and expressions of P-AKT and VEGF in 46 specimens of NSCLCS tissues and 18 specimens of normal lung tissues were detected by SP immunohistochemistry. Their interrelations and correlations were analyzed. Results： Positive rate of P-AKT and VEGF were significantly higher in NSCLC than in normal lung tissues (43.5％ vs 0％ and 67.4% vs 22.2%, P<0.05); there were correlations between PAKT expression and lymph node metastasis, between the TNM stage and differentiation of NSCLC（P<0.05） and between VEGF expression and lymph node metastasis and the TNM stage of NSCLC（P<0.05）. The MVD of lung cancer tissues was higher than that of normal lung tissues(24.62±6.21 vs13.26±5.96, P<0.05); there were correlations between the MVD and lymph node metastasis and between the TNM stage and differentiation of NSCLC（P<0.05）. There were positive correlations between P-AKT expression and VEGF expression and MVD（P<0.05）. Conclusion： P-AKT is over-expressed in NSCLC, which correlates with lymph node metastasis, the TNM stage and differentiation of NSCLC and takes part in the angiogenesis of NSCLC.

Key words: Carcinoma, non-small-cell lung, Protein kinases, Vascular endothelial growth factor, Microvessel density