Abstract: Objective: To explore the role of some special cerebrospinal fluid proteins on the pathogenesis of multiple sclerosis. Methods: Twodimensional gel electrophoresis and peptide mass fingerprinting were used to identify the proteins in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and patients with nonMS inflammatory central nervous system (CNS) disorders. Results: Different twodimentional maps were received because of the above differences. A total of 35 protein spots were identified that were not seen on the control gel. Most of the proteins were known to be present in normal human CSF, and 6 of them had been identified as MS special by others, but only one protein presented in this study. Conclusion: Four proteins have been identified in multiple sclerosis, and their relation should be further studied.

Key words: Multiple sclerosis, Cerebrospinal fluid, Spectrum analysis, mass, Proteome, Electrophoresis, gel, twodimensional