Abstract: To construct a recombinant adenovirus AdMOMP carrying the major outer membrane protein (MOMP) gene of Chlamydia trachomatis serovar E, and provide the basis for further research on Chlamydia trachomatis adenovirus vaccine. Methods: The MOMP gene fragments of Chlamydia trachomatis serovar E were amplified from genome DNA by PCR method. They were cloned into pcDNAII vector and then were subcloneded into the pshuttle vector pDC316. The pshuttle vector pDC316MOMP and the genomic plasmids PBHGlox△E1, 3Cre were cotransfected into 293 cells for package and amplification of recombinant adenovirus AdMOMP, which was identified by PCR and RTPCR.

Key words: Chlamydia trachomatis, Recombinant adenovirus, Genes, major outer membrane protein, Mice, inbred BALB