染料介导光氧化法处理的同种异体动脉体内近期抗钙化性能评价

山东大学学报(医学版)

染料介导光氧化法处理的同种异体动脉体内近期抗钙化性能评价

谷志涛¹，刘鲁祁²

1. 山东大学临床医学院, 山东济南 250012; 2. 山东省千佛山医院心脏外科, 山东济南 250014

收稿日期:2007-03-13 修回日期:1900-01-01 出版日期:2007-10-24 发布日期:2007-10-24
通讯作者: 刘鲁祁

Anti-calcification function property of allogenic arteries cross-linked by dye0mediated photooxidation

GU Zhi-tao¹，LIU Lu-qi²

1. School of Clinical Medicine, Shandong University；2. Department of Cardiovascular Surgery, Shandong Qianfo Shan Hospital

Received:2007-03-13 Revised:1900-01-01 Online:2007-10-24 Published:2007-10-24
Contact: LIU Lu-qi

摘要/Abstract

摘要： 目的：观察染料介导光氧化法（DMP）处理的家兔胸主动脉用于同种异体腹主动脉置换后，在体内血流环境下的抗钙化性能。方法：以经染料介导光氧化反应处理的家兔胸主动脉为研究对象，以戊二醛(GA)处理的家兔胸主动脉以及自体腹主动脉原位移植(OT)作为对照，建立家兔胸-腹主动脉移植模型；实验动物观察饲养1个月后，取出标本，通过原子吸收光谱法测定血管组织钙含量，扫描电镜观察血管壁钙化情况。结果：DMP组血管壁钙含量为(3.58±0.22)μg/mg，GA组血管壁钙含量为(22.5±0.53)μg/mg，自体动脉移植组组织钙含量(2.99±0.33)μg/mg，DMP组组织钙含量虽较OT组高，但差异无统计学意义（P＞0.05），DMP组组织钙含量较GA组低（P＜0.01）；扫描电镜下观察可见，GA组有大量的钙沉积，DMP组仅见少量钙沉积。结论：光氧化反应处理的家兔胸主动脉在家兔胸腹主动脉移植的动物模型中，抗钙化性能优于戊二醛固定的胸主动脉。

关键词: 移植, 抗钙化 , 同种异体动脉, 染料介导光氧化

Abstract: Objective: To study the anti-calcification function property of rabbit thoracic aorta cross-linked by dye-mediated photo-oxidation. Methods: Rabbits whose allogenic thoracic aorta was cross-linked by dye-mediated photo-oxidation (DMP, n=6) were in the DMP group, rabbits whose allogenic thoracic aorta was cross-linked by glutaraldehyde (GA, n=6) were in the GA group, and rabbits whose homogenic abdominal aorta was orthotropically transplanted (OT, n=6) were in the OT group. Then the donor arteries were transplanted in the position of abdominal arteries of rabbits. After operations, animals were fed for 1 month, and then the grafted artery was taken off for observation by HE, SEM and tissue calcium content which was analyzed by flame atomic absorption spectrophotometer. Results: Results of calcium content in the DMP group, the GA group and the OT group was 3.58±0.22μg/mg, 22.5±0.53μg/mg and 2.99±0.33μg/mg, respectively. There was no statistical difference between the DMP group and the OT group (P＞0.05). However, it revealed a significant difference between the DMP group and the GA group (P＜0.01). The calcium content in the DMP group was much lower than that in the GA group. Conclusion: The artery cross-linked by dye-mediated photo-oxidation treatment appears to have less calcification in rabbit allogenic transplantation model.

Key words: Dye-mediated photo-oxidation, Allogenic artery, Transplantation, Anti-calcification

中图分类号:

R654.3

谷志涛,刘鲁祁 . 染料介导光氧化法处理的同种异体动脉体内近期抗钙化性能评价[J]. 山东大学学报(医学版), 2007, 45(10): 1050-1053.

GU Zhi-tao,LIU Lu-qi. Anti-calcification function property of allogenic arteries cross-linked by dye0mediated photooxidation[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 1050-1053.

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