结扎切断法与白芨微粒栓塞法建立大鼠后肢缺血模型效果比较

山东大学学报(医学版)

结扎切断法与白芨微粒栓塞法建立大鼠后肢缺血模型效果比较

梁翠宏¹，田铧²，徐蕴²，李贵宝²，田广平², 宋涛²

1. 潍坊医学院解剖教研室； 2. 山东大学医学院解剖学教研室，山东济南 250012

收稿日期:2006-12-29 修回日期:1900-01-01 出版日期:2007-10-24 发布日期:2007-10-24
通讯作者: 田铧

A comparison of a rat hind-limb ischemia model established by ligation and embolization

LIANG Cui-hong¹，TIAN Hua²，XU Yun²，LI Gui-bao²，TIAN Guang-ping², SONG Tao²

1. Department of Anatomy, Weifang Medical College, Weifang, Shandong, China; 2. Department of Anatomy, School of Medicine, Shandong University, Jinan 250012, Shandong, China

Received:2006-12-29 Revised:1900-01-01 Online:2007-10-24 Published:2007-10-24
Contact: TIAN Hua

摘要/Abstract

摘要： 目的：采用结扎法和白芨微粒栓塞法制作大鼠后肢缺血模型，并对其缺血效果加以比较，以探寻有效的建模方法。方法：取Wistar大鼠分别用结扎股动脉法、结扎髂总动脉法、白芨微粒栓塞法建立大鼠后肢缺血模型。术后1～21天观察大鼠后肢缺血情况及功能改变，取材作病理切片观察。结果：①结扎股动脉或髂总动脉后大鼠后肢仅出现短暂的跛行，组织切片未见异常改变；②白芨微粒栓塞股动脉后，大鼠后肢出现持续跛行、肌肉萎缩和皮肤坏死等表现，组织切片显示大鼠后肢皮肤、肌肉组织均出现明显而持久的缺血或坏死。结论：①结扎血管法不能造成明显的大鼠后肢缺血；②白芨微粒栓塞股动脉法可引起大鼠后肢明显的持续性缺血，是一种较为理想的大鼠后肢缺血模型制作方法。

关键词: 后肢, 大鼠 , 白芨, 栓塞, 结扎法, 缺血模型

Abstract: Objective: To explore an effective method for establishing a hindlimb ischemia model in rats. Methods: Wistar rat hindlimb ischemia models were established by three methods: ligation of the femoral artery, ligation of the common iliac artery, embolization of the femoral artery with fine particles of common bletilla tuber (FPCBT). From 1 to 21 days after operation, the ischemia and function had been observed. Results: ① Temporal limp happened in the operated hindlimbs after ligating the femoral artery or the common iliac artery, and no pathological changes were found in the tissue sections; ② At 3 to 21 days after embolizing the femoral artery by FP-CBT, severe ischemia occurred in the operated hindlimbs, such as sustained limping, skin necrosis or ulcers, and muscle fibers necrosis which was recognized by HE staining. Conclusion: Hindlimb ischemia can not be produced by ligating the artery. However, it can be well induced by embolizing the femoral artery with FP-CBT.

Key words: Ischemia model, Ligation, Common bletilla tuber, Embolization, Hindlimb, Rat

中图分类号:

R332

梁翠宏,田铧,徐蕴,李贵宝,田广平,宋涛 . 结扎切断法与白芨微粒栓塞法建立大鼠后肢缺血模型效果比较[J]. 山东大学学报(医学版), 2007, 45(10): 1008-1010.

LIANG Cui-hong,TIAN Hua,XU Yun,LI Gui-bao,TIAN Guang-ping,SONG Tao. A comparison of a rat hind-limb ischemia model established by ligation and embolization[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 1008-1010.

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