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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (11): 27-31.doi: 10.6040/j.issn.1671-7554.0.2017.142

• 基础医学 • 上一篇    下一篇

植物内生真菌Leptosphaeria sp.中两个新氧杂蒽酮类化合物leptosphaerins H和I的分离及抗炎活性

张鹏亮,张龙腾,李爱玲,沈涛,娄红祥,王小宁   

  1. 山东大学药学院天然产物化学生物学教育部重点实验室, 山东 济南 250012
  • 收稿日期:2017-02-15 出版日期:2017-11-10 发布日期:2017-11-10
  • 通讯作者: 王小宁. E-mail:wangxn@sdu.edu.cn E-mail:wangxn@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(21472113);山东省科技发展计划(2014GSF118023)

Isolation of Leptosphaerins H and I, two new xanthones from the endophytic fungus Leptosphaeria sp. and their anti-inflammatory activity

ZHANG Pengliang, ZHANG Longteng, LI Ailing, SHEN Tao, LOU Hongxiang, WANG Xiaoning   

  1. Key Laboratory of Chemical Biology(Ministry of Education), Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Received:2017-02-15 Online:2017-11-10 Published:2017-11-10

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摘要： 目的 对分离自脚骨脆正常植物组织中的内生真菌Leptosphaeria sp. L1201的代谢产物及其生物活性进行研究。 方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等技术对内生真菌Leptosphaeria sp. L1201的代谢产物进行分离,采用核磁共振、高分辨率质谱、X-射线单晶衍射等技术确证化合物的结构;采用一氧化氮(NO)生成抑制试验评价化合物的抗炎活性,并测定化合物对小鼠RAW264.7巨噬细胞的细胞毒性。 结果 从该株内生真菌培养物的乙酸乙酯提取物中获得2个新的氧杂蒽酮类化合物,并鉴定为leptosphaerins H(1)和I(2);化合物1和2能够抑制小鼠RAW264.7巨噬细胞中NO形成,IC50值分别为126.7 μmol/L和72.6 μmol/L。 结论 Leptosphaerins H(1)和I(2)为新化合物,并在RAW264.7细胞中具有一定的抗炎活性。

关键词: 氧杂蒽酮, 内生真菌, 抗炎活性

Abstract: Objective To study the metabolites from the strain of the fungus Leptosphaeria sp. L1201 which was isolated from healthy tissues of the plant Casearia balansae(Flacourtiaceae). Methods The compounds were isolated by different chromatographic methods, including silica gel and Sephadex LH-20 column chromatography as well as high performance liquid chromatography, and the structures of the compounds were determined on the basis of extensive spectroscopic techniques including HRMS, 1D and 2D NMR experiments and single-crystal X-ray diffraction analysis. Anti-inflammatory effects of isolated compounds were evaluated using nitric oxide(NO)inhibitory assay in murine RAW 264.7 macrophages. Results Two new xanthones were isolated from the EtOAc extract of the culture of the fungus Leptosphaeria sp. L1201 and their structures were identified as leptosphaerins H(1)and I(2). Compounds 1 and 2 inhibited the NO formation with IC50 values being 126.7 and 72.6 μmol/L, respectively. Conclusion Leptosphaerins H and I are new xanthones, which show anti-inflammatory activity in RAW264.7 cells.

Key words: Endophytic fungi, Xanthone, Anti-inflammatory activity

中图分类号: 

  • R914
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