育龄期高水平生殖激素对大鼠围绝经期及绝经后血压的影响

doi:10.6040/j.issn.1671-7554.0.2017.219

摘要/Abstract

摘要： 目的探讨雌性大鼠育龄期体内高水平17-β雌二醇(17-β E₂)或孕酮(P)对其围绝经期及绝经后血压的影响,并观察血管紧张素Ⅱ-1型受体(AT₁R)的变化。方法间情期切除大鼠双侧卵巢,利用17-β E₂和P建立人工动情周期模型,共分为3组:高剂量雌激素加低剂量孕激素组(HELP)、高剂量孕激素加低剂量雌激素组(HPLE)和卵巢切除组(OVX)。经按周期给予17-β E₂和P(8周)、递减剂量(5周)和停止给予(6周)依次模拟自然状态下的育龄期、围绝经期与绝经期,监测血压、血清E₂、P、腹主动脉AT₁R蛋白及其mRNA的表达水平。结果与OVX组比较,HELP组与HPLE组增加各模拟期的收缩压(P<0.05),HELP组明显升高围绝经模拟期与绝经模拟期的舒张压(P<0.05),并增加育龄模拟期和围绝经模拟期AT₁R蛋白及其mRNA的表达(P<0.05);AT₁R蛋白表达水平与血清E₂、SBP呈正相关(P<0.01)。结论育龄期体内高水平的E₂能升高大鼠围绝经期及绝经后血压,且SBP的升高伴随腹主动脉中AT₁R蛋白的高表达。

关键词: 雌激素, 血压, 育龄期, 绝经期, 孕激素, 血管紧张素Ⅱ-1型受体

Abstract: Objective To explore the effects of high-dose endogenous estrogen(17-β E₂)and progesterone(P)of the reproductive phase on the blood pressure of perimenopausal and postmenopausal rats and to observe the role of angiotensin II type-1 receptor(AT₁R). Methods Bilateral ovariectomy was performed during the diestrus phase of estrus cycle to induce rat models of artificial estrous cycle by 17-β E₂ and P. The models were divided into 3 groups: high-dose estrogen plus low-dose progesterone group(n=20, HELP), high-dose progesterone plus low-dose estrogen group(n=20, HPLE), and ovariectomized group(n=20, OVX). The experimental animals were given hormones for 8 weeks, decreasing dosages for 5 weeks and withdrawal hormones for 6 weeks to imitate the reproductive phase, perimenopausal phase and postmenopausal phase respectively. The changes of blood pressure, serum E₂ and P, and protein and mRNA expressions of AT₁R in the abdominal aorta were monitored. Results Compared with the OVX group, the HELP and HPLE groups had higher systolic blood pressure(SBP)in all phases(P<0.05); the HELP group had higher diastolic blood pressure(DBP)in the perimenopausal and postmenopausal phases(P<0.05), and higher protein and mRNA expressions of AT₁R in the reproductive and perimenopausal phases(P<0.05). The protein expression of AT₁R was positively correlated with serum concentration of E₂ and SBP(P<0.01). Conclusion Exposure to excessively high-dose 山东大学学报 (医学版)55卷11期 -贾佳静,等.育龄期高水平生殖激素对大鼠围绝经期及绝经后血压的影响 \=-endogenous estrogen in the reproductive phase raises the blood pressure in perimenopausal and postmenopausal rats. Elevated protein expression of AT₁R in the abdominal aorta is correlated with high SBP.

Key words: Progesterone, Blood pressure, Postmenopausal phase, Estrogen, Reproductive phase, Angiotensin II type-1 receptor

中图分类号:

R574

贾佳静,王志萍. 育龄期高水平生殖激素对大鼠围绝经期及绝经后血压的影响[J]. 山东大学学报（医学版）, 2017, 55(11): 7-14.

JIA Jiajing, WANG Zhiping. Effects of high exposure of endogenous reproductive hormones on perimenopausal and postmenopausal blood pressure in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 7-14.

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