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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (6): 12-15.doi: 10.6040/j.issn.1671-7554.0.2015.855

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下调α-catulin基因的表达对舌鳞癌细胞株Tscca侵袭及迁移能力影响的体外研究

马雪,张斌,韩春耀,刘明媛,郝丽静,葛树卿,薛中原   

  1. 辽宁医学院附属第一医院口腔科, 辽宁 锦州 121001
  • 收稿日期:2015-09-10 出版日期:2016-06-20 发布日期:2016-06-20
  • 通讯作者: 张斌. E-mail:zhangbinjin@sina.com E-mail:zhangbinjin@sina.com
  • 基金资助:
    辽宁省科学技术计划(2015020333)

Down-regulation of α-catulin inhibits the invasion and migration of human tongue squamous cancer cell line Tscca in vitro

MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan   

  1. Department of Stomatology, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2015-09-10 Online:2016-06-20 Published:2016-06-20

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摘要： 目的 探讨α-catulin基因下调对人舌鳞癌细胞株Tscca侵袭、迁移能力的影响。 方法 本实验设为3个组:α-catulin-siRNA组、空质粒转染组和未转染质粒的空白对照组。构建α-catulin沉默质粒转染的人舌鳞癌细胞株Tscca,运用Western blotting检测α-catulin蛋白表达, 采用Transwell及划痕实验检测细胞的侵袭、迁移能力。 结果 Western blotting结果显示,α-catulin-siRNA转染组Tscca细胞α-catulin蛋白表达量减少;Transwell及划痕实验结果显示,α-catulin-siRNA转染组细胞侵袭、迁移能力降低。 结论 下调α-catulin基因的表达可以有效降低人舌鳞癌细胞株Tscca的侵袭、迁移能力,α-catulin有望成为舌癌基因治疗的一个潜在靶点。

关键词: α-catulin, 迁移, 侵袭, 舌癌

Abstract: Objective To explore the effect of α-catulin down-regulation on the invasion and migration of tongue squamous cancer cell line Tscca. Methods Cells were divided into α-catulin-siRNA transfection group, empty plasmid transfection group, and blank group. The α-catulin down-expressing plasmid was constructed and transfected into human tongue squamous cancer cell line Tscca. The protein expression of α-catulin was tested by Western blotting. The in vitro invasion and migration abilities of Tscca cells were measured by Transwell assays and Wound Healing. Results The protein expression of α-catulin reduced remarkably in α-catulin-siRNA trasfection group. Wound-healing and Transwell assays showed that the invasion and migration of Tscca cells were suppressed in α-catulin-siRNA transfection group. Conclusion SiRNA-mediated silencing of the α-catulin gene can significantly inhibit cell invasion and migration in human squamous cell carcinoma line Tscca, indicating that α-catulin might be used as an underlying target for molecular therapy of tongue cancer.

Key words: α-catulin, Migration, Tongue squamous cell carcinoma, Invasion

中图分类号: 

  • R739.86
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