卵母细胞体外成熟评价标准的改良研究

山东大学学报(医学版)

卵母细胞体外成熟评价标准的改良研究

杨永杰¹，孙淑玉²，马水英¹，姜晶晶¹，李梅¹，胡京美¹，李媛¹

山东大学 1. 附属省立医院生殖医学中心，济南 250021； 2. 校医院, 济南 250100

收稿日期:2008-05-06 修回日期:1900-01-01 出版日期:2008-09-16 发布日期:2008-09-16
通讯作者: 李媛

Improvement of the scoring system on human oocytes during maturation in vitro

YANG Yong-jie¹, SUN Shu-yu², MA Shui-ying¹, JIANG Jing-jing¹, LI Mei¹, HU Jing-mei¹, LI Yuan¹

Shandong University 1. Center for Human Reproduction of Shandong Provincial Hospital, Jinan 250021, China； 2. School Hospital, Jinan 250100, China

Received:2008-05-06 Revised:1900-01-01 Online:2008-09-16 Published:2008-09-16
Contact: LI Yuan

摘要/Abstract

摘要： 目的探讨改良式人未成熟卵母细胞评分标准的可行性和颗粒细胞指标的合理性及其对卵子发育潜能的影响。方法体外培养卵丘-卵母细胞复合体，按改良式人未成熟卵母细胞评分标准，将未成熟卵母细胞分为优质卵和非优质卵，对成熟卵母细胞进行受精，并对胚胎进行评价；用琼脂糖凝胶电泳检测两类卵的颗粒细胞凋亡。结果优质卵的成熟率、卵裂率和优质胚胎率都优于非优质卵，优质卵成熟数和优质胚胎数与非优质卵比较差异有统计学意义（P<0.01）；非优质卵其卵丘细胞DNA凝胶电泳后形成明显DNA 梯带，凋亡程度高于优质卵的卵丘细胞。结论改良式人未成熟卵母细胞评分标准在临床上具有可行性。

关键词: 细胞凋亡, 体外成熟, 评价研究, 卵母细胞

Abstract: To study the feasibility of the improved scoring system by evaluating oocytes cultured in vitro, and to discuss the relationship between developmental potential of human immature oocytes and their granulasa cells. Methods Cumulus enclosed oocyte complexes (COCs) were cultured, evaluated and fertilized. The IVM oocytes were morphologically classified into two groups based on the improved scoring standard. One group was of high quality and the other group was of poor quality. Granulosa cell apoptosis was detected by agarose gel electrophoresis. Results The immature oocyte scoring system was improved. The maturation rate，cleavage rate and high quality embryos of high quality oocytes were better than those of poor quality oocytes. DNA of granulosa cells distilled from poor quality oocytes was discovered as DNA ladder，while that from high quality ones was not. ConclusionThe improved scoring standard is feasible in clinical practice.

Key words: Oocytes, In vitro maturation, Scoring system, Apoptosis

中图分类号:

R713.7

杨永杰,孙淑玉,马水英,姜晶晶,李梅,胡京美,李媛 . 卵母细胞体外成熟评价标准的改良研究[J]. 山东大学学报(医学版), 2008, 46(9): 869-872.

YANG Yong-jie,SUN Shu-yu,MA Shui-ying,JIANG Jing-jing,LI Mei,HU Jing-mei,LI Yuan. Improvement of the scoring system on human oocytes during maturation in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(9): 869-872.

参考文献

相关文章 15

[1]	鹿向东杨伟徐广明曲元明. 脑膜瘤中PPAR-γ的表达及曲格列酮对脑膜瘤培养细胞生长的影响[J]. 山东大学学报(医学版), 2209, 47(6): 65-.
[2]	李响,张艺,王雪纯,徐梦超,王月兰. 氧化应激在创伤性脑损伤诱发急性肺损伤中的研究进展[J]. 山东大学学报 (医学版), 2025, 63(2): 118-124.
[3]	姜子晗,芦兴晨,孙露,赵蕙琛,左丹,马小莉,刘元涛,张玉超. NR4A1通过IκBα/NF-κB通路调控过氧化氢诱导人脐静脉内皮细胞凋亡的机制[J]. 山东大学学报 (医学版), 2024, 62(3): 11-19.
[4]	刘金波,刘凯文,向崇鑫,程雷. 西红花苷对椎间盘退变的保护作用[J]. 山东大学学报 (医学版), 2023, 61(9): 84-93.
[5]	杨元凤,熊高才,黎豫川,罗玉玲,张敬杰. 鹿苓安肾颗粒对慢性肾功能衰竭大鼠炎症反应及细胞凋亡的影响[J]. 山东大学学报 (医学版), 2023, 61(10): 9-16.
[6]	马翔,赵飞燕. 。卵子冷冻保存现状与进展[J]. 山东大学学报 (医学版), 2022, 60(9): 19-23.
[7]	赵舸,邹存华,宋冬冬,赵淑萍. 丹参酮IIA对子宫内膜癌细胞增殖与凋亡的影响[J]. 山东大学学报 (医学版), 2022, 60(9): 53-58.
[8]	封海岗,刘国文,曹洪. 干扰MAD2L1基因表达对乳腺癌细胞凋亡的影响及机制[J]. 山东大学学报 (医学版), 2022, 60(10): 9-16.
[9]	卢游,且迪,伍晋辉,杨凡. 干预Sonic Hedgehog信号通路对宫内发育迟缓新生大鼠学习记忆能力的影响[J]. 山东大学学报 (医学版), 2021, 59(5): 82-89.
[10]	薛源,林雪艳,徐歌,田永杰. 低氧诱导因子-1α在子宫内膜异位症患者血清中的表达和对在位子宫内膜间质细胞上皮-间质转化的影响[J]. 山东大学学报 (医学版), 2021, 59(2): 41-47.
[11]	张晓璐,王丽莉,陈凯明,娄宪芝,张曼. 组蛋白去乙酰化酶SIRT1经Toll样受体4途径对巨噬细胞凋亡的调控[J]. 山东大学学报 (医学版), 2020, 58(12): 8-14.
[12]	史丽,马静,赵喜娃,关英霞,赵连梅,单保恩. miR-25-3p在40例子宫内膜腺癌组织中的表达及对KLE细胞生物学功能的影响[J]. 山东大学学报 (医学版), 2020, 58(12): 86-91.
[13]	张真真,韩婷,李敬,石玉华. 卵巢抵抗综合征不孕患者行卵子体外成熟培养后冻胚移植足月分娩1例[J]. 山东大学学报 (医学版), 2019, 57(2): 117-119.
[14]	阮祥燕,程姣姣,杜娟,李扬璐,金凤羽,Alfred O. Mueck. 生育力保护方法研究进展[J]. 山东大学学报 (医学版), 2019, 57(2): 29-34.
[15]	毕涛,卢倩倩,孙传东. 腺病毒介导HBx对人淋巴细胞影响的体外实验[J]. 山东大学学报 (医学版), 2018, 56(7): 15-20.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed