创伤性应激对大鼠海马BDNF表达的影响

doi:10.6040/j.issn.1671-7554.0.2015.500

摘要/Abstract

摘要： 目的探讨精神创伤后应激大鼠海马组织中脑源性神经营养因子(BDNF)的水平变化及其意义。方法将Wistar大鼠随机分为正常组与应激组(应激造模后1 d组、7 d组、14 d组),每组8只。应激组建立SPS&S模型,采用旷场实验、Morris水迷宫等方法检验各组大鼠造模前后的行为学指标变化并评估造模效果,采用酶联免疫吸附法(ELISA)测定各组大鼠海马组织中 BDNF蛋白的水平变化。结果各组两两比较,正常组海马组织中BDNF水平高于14 d组,差异有统计学意义(Z=-3.363,P=0.001),与1 d组和7 d组无统计学差异;应激各组之间两两比较无统计学差异。结论正常组海马BDNF含量高于应激组(14 d组),应激后BDNF的水平有所下降,但其变化过程是缓慢的,BDNF可能成为精神创伤后应激的生物学标志之一。

关键词: 脑源性神经营养因子, 应激, 酶联免疫吸附法

Abstract: Objective To investigate changes of brain-derived neurotrophic factor(BDNF)in the hippocampus of rats suffering from post-traumatic stress, and to explore the significance of such changes. Methods A total of 32 Wistar rats were randomly divided into control group and post-traumatic stress group, which was subdivided into 3 groups:1 d group(1 day after modeling, n=8), 7 d group(7 days after modeling, n=8), and 14 d group(14 days after modeling, n=8). Rats in the stress groups were evaluated with open-field test and Morris water maze, and changes in the ethological indexes before and after modeling were assessed, followed by determination of BDNF level in hippocampus of the each group of rats by ELISA. Results BDNF level of the 14 d group was significantly lower than that of the control(Z=-3.363, P=0.001), but there was no difference between the 1 d group and control, 7 d group and control, and 1 d group and 7 d group. Conclusion BDNF level decreases in rats suffering from post-traumatic stress, but such change is rather slow. BDNF is a potential marker for post-traumatic stress.

Key words: Stress, Brain-derived neurotrophic factor, Enzyme linked immunosorbent assay

中图分类号:

R749.5

袁秀玉,董原君,梁霞,胡敏,张桂青. 创伤性应激对大鼠海马BDNF表达的影响[J]. 山东大学学报（医学版）, 2016, 54(4): 37-41.

YUAN Xiuyu, DONG Yuanjun, LIANG Xia, HU Min, ZHANG Guiqing. Effect of post-traumatic stress on hippocampal BDNF expression in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 37-41.

参考文献

[1] 沈渔邨. 精神病学[M]. 5版. 北京: 人民卫生出版社, 2009:620-627.
[2] 李晓照, 陈泽奇, 胡随瑜, 等. 抑郁症患者血清 BDNF水平与相关因素分析[J]. 中国临床心理学杂志, 2005, 11(13): 312-314. LI Xiaozhao, CHEN Zeqi, HU Suiyu, et al. An analysis of serum levels of BDNF and relative factors in depressive patients[J]. Chinese Journal of Clinical Psychology, 2005, 11(13):312-314.
[3] Zhang L, Zhou R, Li X, et al. Stress-induced change of mitochondria membrane potential regulated by genomic and non-genomic GR signaling: A possible mechanism for hippocampus atrophy in PTSD[J]. Med Hypothese, 2006, 66(6):1205-1208.
[4] 邵淑红, 李尊岭, 潘芳, 等. 急、慢性应激对大鼠海马 BDNF表达的影响[J]. 基础医学与临床, 2012, 32(4):428-432. SHAO Shuhong, LI Zunling, PAN Fang, et al. Effect of acute and chronic stress on the expression of BDNF and BDNF mRNA in the hippocampus of rats[J]. Basic & Clinical Medicine, 2012, 32(4):428-432.
[5] 李功迎,李凌江,孔媛. 脑源性神经营养因子与应激[J]. 中国行为医学科学, 2005, 14(12):1134-1135. LI Gongying, LI Lingjang, KONG Yuan. Brain-derived neurotrophic factor and stress[J]. Chin J of Behavioral Med Sci, 2005, 14(12):1134-1135.
[6] 毛萌, 宋月晗, 李峰, 等. SPS 建立 PTSD 样大鼠旷场行为的改变及十味温胆汤的调节作用[J].现代生物医学进展, 2013, 13(17): 3214-3216. MAO Meng, SONG Yuehan, LI Feng, et al. Effects of shiweiwendan soup on the behavior responses of PTSD rats established by SPS in the open field[J]. Progress in Modern Biomedicine, 2013, 13(17):3214-3216.
[7] 赵中兴, 伍亚民. 一种新的大鼠创伤后应激障碍模型的建立及其行为学检测[J]. 第三军医大学学报, 2012, 10(32):928-932. ZHAO Zhongxing, WU Yamin. Establishment of a new rat model of posttraumatic stress disorder and its behavior test[J]. J Third Mil Med Univ, 2012, 10(32):928-932.
[8] 范琼尹, 薛瑞, 李洋, 等. 单程延迟性应激诱导雌性大鼠创伤后应激障碍模型的建立[J]. 中国药理学和毒理学杂志, 2013, 4(27):715-718. FAN Qiongyi, XUE Rui, LI Yang, et al. One-way stress induced delayed female rats with post-traumatic stress disorder model[J]. Chin J Pharmacol Toxicol, 2013, 4(27): 715-718.
[9] 隋竹欣, 刘昊, 王海涛, 等. 创伤后应激障碍大鼠海马神经元凋亡和Akt/mTOR信号通路的改变[J]. 四川大学学报: 医学版, 2014, 45(2):221-224. SUI Zhuxin, LIU Hao, WANG Haitao, et al. Alternation of apoptosis and Akt/mTOR signal pathway in hippocapal neurons of rat with post-traumatic stress [J]. J Sichuan Univ: Med Sci Edi, 2014, 45(2): 221-224.
[10] 唐佳文, 李想, 邢雪松. bFGF 对 PTSD样大鼠海马组织ERK表达的影响[J]. 沈阳医学院学报, 2015, 1(17):53-55. TANG Jiawen, LI Xiang, XING Xuesong. Effect of post-traumatic stress on hippocampal bFGF ERK expression in rat[J]. Journal of Shenyang Medical College, 2015, 1(17):53-55.
[11] Green CR, Corsi-Travali S, Neumeister A. The role of BDNF-TrkB signaling in the pathogenesis of PTSD[J]. J Depress Anxiety, 2013(S4): pii:006.
[12] 符卫仙, 唐牟尼, 黄悦勤, 等. 强迫症血清脑源性神经营养因子水平的研究[J]. 广州医药, 2011, 42(2):1-3. FU Weixian, TANG Mouni, HUANG Yueqin, et al. A study on serum brain- derived neurotrophic factor levels in patients with obsessive- compulsive disorder[J]. Guangdong medicine, 2011, 42(2):1-3.
[13] 翟杰, 薛波, 仇新华, 等. 学习障碍患儿血清BDNF水平的研究[J]. 精神医学杂志, 2007, 20(1):8-9. ZHAI Jie, XUE Bo, QIU Xinhua, et al. Serum brain-derived neurotrophic factor(BDNF)levels in children with learning disorder[J]. Journal of Psychiatry, 2007, 20(1):8-9.
[14] 陈大春, 李艳丽, 修梅红, 等. 利培酮治疗首发精神分裂患者血清脑源性神经营养因子的变化及其与临床疗效和认知功能的关系[J]. 中国神经精神疾病杂志, 2010, 36(9):529-532. CHEN Dachun, LI Yanli, XIU Meihong, et al. Effect of risperidon treatment on serum levels of BDNF and the correlation between serum BDNF levels and clinical efficiency and cognition improvement in first- episode schizophrenic patients[J]. Chin J Nerv Ment Dis, 2010, 36(9):529-532.
[15] 苏珊珊, 肖泽萍, 林志光, 等. PTSD患者血浆BDNF水平[C]. 国际创伤与应激学术会议暨浙江省医学会行为医学分会第二届学术年会论文汇编, 2014.
[16] Wang Z, Li Z, Chen J, et al. Association of BDNF gene polymorphism with bipolar disorders in Han Chinese population[J]. Genes Brain Behav, 2012, 11(5):524-528.
[17] Monteleone P, Serritella C, Martiadis V, et al. Decreased levels of serum brain-derived neurotrophic factor in both depressed and euthymic patients with unipolar depression and in euthymic patients with bipolar I and II disorders [J]. Bipolar Disord, 2008, 10(1):95-100.
[18] Rakofsky JJ, Ressler KJ, Dunlop BW. BDNF function as a potential mediator of bipolar disorder and post-traumatic stress disorder comorbidity[J]. Mol Psychiatry, 2012, 17(1):22-35.
[19] Scaccianoce S, Del Bianco P, Caricasole A, et al. Relationship between leaning, study, stress and hippcampal brain-derived neurotrophic factor [J]. Neuroscience, 2003, 121:825-828.
[20] Scheepens A, Wassink G, Blanco CE. The effect of a global birth asphyxia on the ontogeny of BDNF and NGF protein expression in the juvenile brain[J]. Brain Res Dev Brain Res, 2003, 140:215-221.
[21] Adlard PA, Cotman CW. Voluntary exercise protects against stress-induced, decreases in brain-derived neurotrophic factor protein expression[J]. Neuroscience, 2004, 124:985-992.

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