抵抗素样分子 β在动脉粥样硬化斑块稳定性中的作用

doi:10.6040/j.issn.1671-7554.0.2015.1137

摘要/Abstract

摘要： 目的探讨抵抗素样分子β(RELMβ)在动脉粥样硬化易损斑块模型中的作用。方法 60只雄性8周龄ApoE-/-小鼠通过高脂饮食及颈总动脉套管诱导易损斑块形成,进而斑块局部转染慢病毒,根据转染慢病毒的不同随机分为正常对照组、转染携带空载体的慢病毒组(si-NC组)和转染携带干扰序列的慢病毒组(si-RELMβ组),每组20只。继续高脂喂养4周,称重后行安乐死,留取空腹血清及颈总动脉标本。检测血清中血脂含量。采用Western blotting法检测斑块内RELMβ蛋白表达水平。采用天狼猩红及油红O染色分别检测斑块内胶原和脂质含量。采用免疫组化染色法检测斑块内平滑肌细胞、巨噬细胞、白介素-1β(IL-1β)和白介素-6(IL-6)的表达。结果 3组小鼠体质量及血脂水平无明显差异。si-RELMβ组RELMβ蛋白表达较正常对照组显著降低(P<0.05)。si-RELMβ组斑块内胶原和平滑肌细胞含量较正常对照组显著增加,而脂质和巨噬细胞含量却显著减少,易损指数较正常对照组亦明显降低(P<0.05)。si-RELMβ组炎症因子——IL-1β和IL-6较正常对照组显著减少(P<0.05)。结论 RELMβ能够促进动脉粥样硬化易损斑块的形成,其机制可能与其促进斑块内炎症因子表达有关。

关键词: 动脉粥样硬化, 抵抗素样分子 β, 白介素-6, 炎症反应, 白介素-1β

Abstract: Objective To explore the effect of resistin-like molecule β(RELMβ)on the vulnerability of atherosclerotic plaques in ApoE-/- mice. Methods A total of 60 male ApoE-/- mice(8 weeks old)were fed with a high-fat diet and a constrictive silastic tube was placed to induce the vulnerable plaques. All mice were divided into 3 groups after surgery(n=20 per group): control, si-NC and si-RELMβ. At the end of week 14, ApoE-/- mice were euthanized to collect the right common carotid arteries and blood from the left ventricle. The contents of total cholesterol, triglycerides, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol in the blood samples were detected. The protein expressions of RELMβ in all mice were determined with Western blotting. The collagen and lipoid depositions in the plaques were determined with collagen sirius red staining and oil-red O staining, respectively. The contents of macrophages, smooth muscle cells(SMCs), interlenkin-1 beta(IL-1β)and interlenkin-6(IL-6)expressions in the plaques were detected with immunohistochemical methods. Results There were no significant differences in body weight and lipid levels among the 3 groups. Compared with the control group, the si-RELMβ group had significantly decreased RELMβ protein expression, lipid, macrophages, vulnerable index, and expressions of IL-1β and IL-6, increased contents of collagen and SMCs(P<0.05), while those in the si-NC group had no statistical differences. Conclusion RELMβ can increase atherosclerotic plaque instability, and its mechanism is involved with the elevated expressions of IL-1β and IL-6.

Key words: Inflammation, Interlenkin-6, Atherosclerosis, Resistin-like molecule β, Interlenkin-1 beta

中图分类号:

R541.4

孙鹏飞,孟晓,张凯,黎莉. 抵抗素样分子 β在动脉粥样硬化斑块稳定性中的作用[J]. 山东大学学报（医学版）, 2016, 54(3): 1-4.

SUN Pengfei, MENG Xiao, ZHANG Kai, LI Li. The effect of resistin-like molecule β on the vulnerability of atherosclerotic plaques in ApoE-/- mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 1-4.

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