柴朴汤调控VEGF、TGF-β1的表达对哮喘大鼠气道重塑的影响

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (S1): 14-17.

柴朴汤调控VEGF、TGF-β1的表达对哮喘大鼠气道重塑的影响

黄艳¹, 旷昕², 熊花³, 刘鑫¹, 罗晓青³, 刘斌³

1. 南华大学附属第一医院中医科, 湖南衡阳 421001;
2. 南华大学附属第一医院麻醉科, 湖南衡阳 421001;
3. 南华大学, 湖南衡阳 421001

收稿日期:2014-11-30 发布日期:2014-12-30
基金资助:
湖南省中医药管理局资助课题(2010088)

Effects of Chaipu decoction on expression of VEGF and TGF-β1on airway remodeling in asthmatic rats

HUANG Yan¹, KUANG Xin², Xiong Hua³, LIU Xin¹, LUO Xiao-qin³, LIU Bin³

1. Department of Traditional Chinese Medicine, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
2. Department of Anesthesiology, the First Hospital Affiliated to University of South China, Hengyang Hunan 421001, China;
3. University of South China, Hengyang Hunan 421001, China

Received:2014-11-30 Published:2014-12-30
Contact: 旷昕, gdl0422@qq.com E-mail:gdl0422@qq.com

摘要/Abstract

摘要： 目的探讨柴朴汤治疗哮喘的作用机制及与血管内皮生长因子(VEGF)、气道上皮分泌转化生长因子-β1(TGF-β1)表达的关系。方法 40只大鼠随机分成4组: 正常对照组(A组)、地塞米松干预组(B组)、柴朴汤干预组(C组)、模型组(D组),每组 10只。采取苏木精-伊红染色观察气道重塑,RT-PCR方法检测肺部VEGF、TGF-β1mRNA表达,免疫组化方法检测气道壁VEGF、TGF-β1蛋白表达。结果 D 组显示支气管上皮组织增生,支气管平滑肌肥大,周围可见大量炎性细胞浸润,并伴有新生血管的形成,管腔变窄,管壁及基底膜增厚,管腔内可见黏液栓。D组大鼠VEGF和TGF-β1的mRNA相对灰度值均为最高(P<0.05),VEGF和TGF-β1的蛋白的积分光密度值(IOD)均为最高(P<0.01),组均为最低(P<0.05,P<0.01);B、C组间表达差异无统计学意义(P>0.05),但低于D组。结论哮喘大鼠VEGF、TGF-β1表达水平增高,VEGF、TGF-β1可能参与了哮喘大鼠的气道重塑。早期使用柴朴汤治疗可下调VEGF、TGF-β1蛋白的表达,从而减轻气道重塑。

关键词: 柴朴汤, 安全型留置采血针, 哮喘, 气道重塑, 静脉采血, 血管内皮生长因子, 真空采血管, 转化生长因子-β1

Abstract: Objective To investigate the machnism of Chaipu decoction in treatment of asthma and its effects on expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Methods Forty SD rats randomly divided into four groups: the control group (group A), the dexamethasone group (group B), the ChaiPu decoction group (group C), the asthma chronic model group (group D). Airway remodeling was observed by H&E staining. Expression of VEGF, TGF-β1 mRNA and protein were measured by RT-PCR and immune histochemical method, respectively. Results Inflammatory cell infiltration, angiogenesis, thickening of the epithelial basement membrane and bronchial wall and bronchial narrowing were observed in D model group. The results of RT-PCR semi-quantitative analysis showed that gray value of VEGF, TGF-β1 of group D were significantly higher than other groups(P<0.05). The observed value of integral optical density (IOD) of VEGF, TGF-β1 were highest in group D (P<0.01) and lowest in group A (P<0.05 or P<0.01). Conclusion The increased expression of VEGF and TGF-β1 in asthmatic rats suggest that VEGF and TGF-β1 may be involved in airway modulating. Early use of Chaipu decoction in the treatment of asthma may down-regulate VEGF, TGF-β1 expression, therefore inhibit airway remodeling.

Key words: Chaipu decoction, VEGF, TGF-β1, Airway remodeling, Asthma

中图分类号:

R361

黄艳, 旷昕, 熊花, 刘鑫, 罗晓青, 刘斌. 柴朴汤调控VEGF、TGF-β1的表达对哮喘大鼠气道重塑的影响[J]. 山东大学学报（医学版）, 2014, 52(S1): 14-17.

HUANG Yan, KUANG Xin, Xiong Hua, LIU Xin, LUO Xiao-qin, LIU Bin. Effects of Chaipu decoction on expression of VEGF and TGF-β1on airway remodeling in asthmatic rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(S1): 14-17.

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