皮肤T细胞淋巴瘤相关抗原5在肿瘤细胞株中的表达

doi:10.6040/j.issn.1671-7554.0.2014.618

摘要/Abstract

摘要： 目的探讨皮肤T细胞淋巴瘤相关抗原5(cTAGE5)在肿瘤细胞株中的表达及其在肿瘤发生发展中的生物学作用。方法细胞免疫荧光化学检测cTAGE5来源于人的肿瘤细胞株的表达。取阳性表达cTAGE5的人卵巢透明细胞癌ES-2细胞, 分为正常对照组、脂质体对照组、siRNA转染组、表柔比星(终浓度1×10^-6mol/L)对照组,平板克隆形成试验和MTT法观察cTAGE5对细胞生长增殖能力的影响, 细胞划痕实验检测细胞迁移能力。结果 cTAGE5在乳腺癌、肺癌、卵巢癌均有表达, 而在胃癌和肝癌肿瘤细胞中表达不明显。与正常对照组比较, siRNA转染组的肿瘤细胞增殖生长抑制(P<0.01), 细胞迁移能力降低(P<0.01), 表柔比星对照组也显示肿瘤细胞增殖抑制(P<0.01), 细胞迁移能力降低(P>0.01)。结论 cTAGE5在不同组织来源的肿瘤细胞株表达存在差异, siRNA干扰cTAGE5表达可有效抑制肿瘤细胞的增殖和迁移能力。

关键词: 迁移, 皮肤T细胞淋巴瘤相关抗原5, 肿瘤细胞, RNA干扰, 增殖

Abstract: Objective To investigate the expression of the cutaneous T-cell lymphoma-associated antigen 5(cTAGE5) in tumor cell lines and its biological role in the development of tumor preliminarily. Methods Expression of cTAGE5 in tumor cell lines was detected by immunofluorescence. Ovarian clear cell adenocarcinoma cell line ES-2, which was proved to be positive expression of cTAGE5, were divided into normal control group, liposome control group, siRNA transfection group and epirubicin control group(final concentration 1×10^-6mol/L). The Proliferation and growth ability of ES-2 were detected by plate clone formation test and MTT test respectively, and migration ability was detected by cell scratch assay. Results cTAGE5 was expressed in cell lines got from human breast cancer, lung cancer and ovarian cancer, but little in cell lines from human stomach and liver cancer. Compared with normal control group, growth and proliferation ability of tumor cells in siRNA transfection group decreased(P<0.01), migration ability reduced (P<0.01), and proliferation ability of tumor cells in epirubicin control group decreased(P<0.01), also migration ability reduced (P<0.01). Conclusion Expression of cTAGE5 in tumor cell lines from different tissue was different. Inhibition of cTAGE5 expression can depress the proliferation, growth and migration ability of tumor cells.

Key words: Migration, Cutaneous T-cell lymphoma-associated antigen 5, Tumor cells, Proliferation, RNA interfering

中图分类号:

R966

高峰, 康白, 杨洪鸣, 赵廷坤, 曲梅花, 王金红. 皮肤T细胞淋巴瘤相关抗原5在肿瘤细胞株中的表达[J]. 山东大学学报（医学版）, 2015, 53(3): 41-45.

GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong. Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 41-45.

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