关键词: 地西他滨, 活性氧离子, G0/G1期, 线粒体

Abstract: Objective  To investigate the effect of decitabin (DAC) on the mitochondrial biogenesis of synchronized G0/G1 cells. Methods  MDS-L cells were treated with aphidicolin (APC) to synchronize cell cycle at G0/G1 stage. After treatment of decitabine at different concentrations (0, 5, 10, 15μmol/L), reactive oxygen species (ROS) productions were detected by DCFH-DA. Changes of mitochondrial DNA copy number and expressions of coded genes,NADH dehydrogenase 1 (ND1) and NADH dehydrogenase 6(ND6), were detected with qRT-PCR.  Results  In comparison with the control group, decitabine at low concentration (5μmol/L) could promote the production of ROS (P<0.05) and increase the copy number of mtDNA (P<0.05); however, as the concentration increased to 15μmol/L, ROS production started to decline to even lower than that of the control (P<0.05). Besides, DAC could significantly change the expressions of ND1 and ND6 at high concentration. Conclusion  Decitabine can affect mitochondrial biogenesis by altering the mtDNA copy number and gene expressions in a concentration-dependent manner.

Key words: G0/G1 stage, Mitochondria, Reactive oxygen species, Decitabine