关键词: 玻璃化冷冻, 卵母细胞, 受精, 体外成熟, 微丝, 人

Abstract: Objective  To find the changes of development potential and microfilament structure which were affected by vitrification for human in vitro matured oocytes. Methods  Human in vitro matured oocytes were gathered and randomly divided into two groups: fresh group and vitrified group. The oocytes were fertilized and immunofluorescencently stained. The numbers of 2PN, blastocyst and high quality blastocyst were calculated and the microfilaments structures were observed by laser confocal microscopy (LSCM). Results  The survival rate of oocytes was 83.74% after vitrification-warming. The 2PN, blastocyst and high quality blastocyst of the fresh group were significantly higher than those of vitrified group (P<0.05). There was no statistical difference in normal microfilaments rate between fresh group and vitrified group (71.67% vs 67.50%, P=0.71). Conclusion  The proper vitrification-warm process doesn’t change the microfilaments structures of human in vitro matured oocytes, but it reduces their development potential. Thus, morphology of the microfilaments alone can’t be taken as the indicator of the developmental potential.

Key words: In vitro maturation, Vitrification, Human, Microfilament, Fertilization, Oocyte