自发性高血压大鼠心肌声学密度与心肌纤维化的关系及药物干预研究

山东大学学报(医学版)

自发性高血压大鼠心肌声学密度与心肌纤维化的
关系及药物干预研究

王静¹，杜乃立²，赵卓²，郝恩魁²，相有章³

（1. 山东大学医学院，济南 250012； 2.山东大学附属济南市中心医院心内科，济南 250013；3. 山东省地方病防治研究所克山病科，济南 250014）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-16 发布日期:2009-01-16
通讯作者: 杜乃立

Correlation between acoustic densitometry and myocardial fibrosis and effect of drug intervention on them

WANG Jing¹， DU Naili²， ZHAO Zhuo²， HAO Enkui², XIANG Youzhang³

（1. School of Medicine, Shandong University, Jinan 250012, China;
2.Department of Cardiology, Jinan Central Hospital, Shandong University, Jinan 250013, China; 3.Shandong Provincial Institute for Endemic Disease Control, Jinan 250014, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-16 Published:2009-01-16
Contact: DU Naili

摘要/Abstract

摘要： 目的探讨声学密度定量分析（AD）技术评估高血压心肌纤维化的价值，同时观察苯那普利和缬沙坦对高血压心肌纤维化的作用。方法10周龄的雄性自发性高血压大鼠（SHR）60只，随机分为SHR对照组(SHRc组)、SHR苯那普利组(SHRb组)、SHR缬沙坦组(SHRv组)和SHR联合用药组(SHRb+v组)，每组15只。用药组分别给予苯那普利、缬沙坦或二者半剂量合用灌胃10周，并以Wistar京都（WKY）大鼠15只为正常对照(WKY组)。应用AD技术获取各组大鼠左室相应部位心肌背向散射积分（IBS）与背向散射积分周期变化值（CVIB）；测定其收缩压（SBP）、左室重量（LVM）以及左室重量指数（LVMI）和左室心肌羟脯氨酸浓度（HC）；Masson染色观察心肌胶原含量变化，测量心肌胶原容积分数（CVF）和心肌内血管周围胶原面积（PVCA）,并对所得指标进行相关分析。结果与WKY组相比，SHRc组大鼠SBP、LVM、LVMI、HC、CVF、PVCA、IBS均明显升高（P<0.01），而CVIB明显降低(P<0.01),三个用药组以上指标均明显改善（P<0.05, P<0.01）。除血压外，其他指标均以联合用药组改善最为显著（P<0.05, P<0.01）。相关分析显示，HC与CVF及PVCA均呈显著正相关（r=0.921、0.885,P<0.01）。室间隔及左室后壁IB%值与CVF（r=0.731、0.756，P<0.01）及PVCA(r=0.776、0.788，P<0.01)均呈显著正相关，而CVIB值与CVF（r=-0.642、-0.716，P<0.01）及PVCA（r=-0.720、-0.755,P<0.01）均呈显著负相关。结论AD技术是评估高血压心肌纤维化及其严重程度的一项可靠技术。苯那普利和缬沙坦可显著降低血压、减轻自发性高血压大鼠早期心肌纤维化，且二者合用可明显减轻心肌纤维化程度。

关键词: 高血压, 自发性高血压大鼠, 声学密度, 心肌纤维化

Abstract:

To evaluate the quantitative acoustic densitometry (AD) technique in myocardial fibrosis diagnosis and to explore the effects of benazepril and valsartan on myocardial fibrosis. MethodsSixty tenweekold male spontaneously hypertensive rats (SHRs) were randomly assigned to the control group(SHRc group), the benazepril group(SHRb group), the valsartan group(SHRv group) and the combination group(SHRb+v group), which were subjected to an intragastric administration for ten weeks. Fifteen male agematched WistarKyoto (WKY) rats served as the control group. The integrated backscatter (IBS) and cyclic variation of the integrated backscatter (CVIB) were assessed with the AD technique. The cardiac collagen fraction(CVF) and perivascular circumferential area(PVCA) were determined by Masson staining. Myocardial hyproconcentration(HC) were measured by a spectrophotometric method. Also, systolic blood pressure(SBP), left ventricular mass (LVM) and left ventricular mass index(LVMI) were observed. Correlation analysis among the above indexes was analyzed. ResultsCompared with the WKY group, levels of SBP, LVM, LVMI, HC, CVF, PVCA and IBS were all significantly increased in the SHRc group(P<0.01), while CVIB was significantly decreased(P<0.01), and the indexes were substantially ameliorated in the three treated SHR groups(P<0.05, P<0.01). Except for SBP, the combination group showed a more pronounced amelioration than the other two monotherapy groups (P<0.05, P<0.01). An intimately positive correlation was found between HC and CVF(r=0.921, P<0.01)　or PVCA　(r=0.885, P<0.01). Normalized values of integrated backscatter at the interventricular septum and left ventricular posterior wall were positively related to CVF　(r=0.731, 0.756，P<0.01)　and PVCA　(r=0.776, 0.788，P<0.01), while both CVIBs were inversely related to CVF（r=-0.642,-0.716，P<0.01）and PVCA　（r=-0.720, -0.755, P<0.01）. ConclusionMyocardial ultrasonic backscatter parameters are closely correlated with the alteration of myocardial collagen contents. Quantitative myocardial acoustic densitometry is a reliable technique for detecting myocardial fibrosis and evaluating its severity. Benazepril and valsartan decrease the blood pressure and can reverse an early myocardial fibrosis in SHRs. Benazepril in combination with valsartan is more effective in ameliorating myocardial fibrosis than either one alone.

Key words: Hypertension, Spontaneously hypertensive rat, Acoustic densitometry, Myocardial fibrosis

中图分类号:

R541.3

王静1 ，杜乃立2 ，赵卓2 ，郝恩魁2 ，相有章3
. 自发性高血压大鼠心肌声学密度与心肌纤维化的
关系及药物干预研究[J]. 山东大学学报(医学版), 2009, 47(01): 50-55.

WANG Jing¹， DU Naili²， ZHAO Zhuo²， HAO Enkui², XIANG Youzhang³
. Correlation between acoustic densitometry and myocardial fibrosis and effect of drug intervention on them[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(01): 50-55.

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