谷胱甘肽对KLE与HEC1b子宫内膜癌细胞生长的影响

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (9): 58-61.

谷胱甘肽对KLE与HEC1b子宫内膜癌细胞生长的影响

张培海¹，丁宁¹，孔峰²，毕文祥²

山东大学 1. 齐鲁医院妇产科, 济南 250012; 2. 医学院生物化学与分子生物学研究所，济南 250012

收稿日期:2009-02-22 发布日期:2009-09-16
通讯作者: 毕文祥(1962- ),男,副教授,博士,主要从事肿瘤分子生物学研究。Email:biwenxiang@sdu.edu.cn
作者简介:张培海(1965- ),男,副主任医师,博士,主要从事妇科肿瘤研究。

Effect of glutathione on proliferation of KLE and 
HEC1b endometrial cancer cells

ZHANG Peihai¹,　DING Ning¹,　KONG Feng²,　BI Wenxiang²

1. Department of Obstetrics and Gynecology, Qilu Hospital of Shangdo ng University, Jinan 250012, China；
2. Institute of Biochemistry and Molacular Biology, School of Medicine, Shandong University, Jinan 250012, China

Received:2009-02-22 Published:2009-09-16

摘要/Abstract

摘要：

目的比较还原型谷胱甘肽（GSH）对KLE和HEC1b子宫内膜癌细胞生长的影响，并探讨其机制。方法①用GSH处理KLE和HEC1b子宫内膜癌细胞，噻唑蓝（MTT）法检测两型细胞的增殖水平；②用5 mmol/L GSH处理KLE和HEC1b子宫内膜癌细胞，流式细胞仪检测细胞内活性氧（ROS）水平，酶标仪检测细胞内GSH和氧化型谷胱甘肽（GSSG）水平，分光光度计检测细胞内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSHPx）活性。结果在不进行处理的情况下，KLE子宫内膜癌细胞较HEC1b子宫内膜癌细胞的ROS水平及CAT活性较低，GSH水平、GSH/GSSG比值及SOD和GSHPx活性较高（P＜0.01）。用GSH处理后，KLE子宫内膜癌细胞生长加快，细胞内ROS和GSH水平及GSH/GSSG比值下降，SOD、CAT和GSHPx活性降低（P＜0.01）；HEC1b子宫内膜癌细胞生长被抑制，细胞内ROS、GSH及GSSG水平下降，GSH/GSSG比值明显升高，CAT和GSHPx活性降低（P＜0.01）。结论GSH对两种子宫内膜癌细胞生长有明显不同的影响，与两种细胞内ROS水平和抗氧化系统活性有密切的关系。

关键词: 子宫内膜肿瘤, 活性氧, 谷胱甘肽, 抗氧化酶

Abstract:

To investigate the effects of glutathione on proliferation of typeⅠand Ⅱ endometrium cancer cells and to explore its related mechanism. Method① TypeⅠand Ⅱ endometrium cancer cells were challenged by GSH, and proliferation of cells was observed with MTT assay. ② TypeⅠor Ⅱ endometrium cancer cells were treated with 5?mmol/L GSH and the cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined. ResultsWithout treatment with GSH, typeⅠ endometrium cancer cells, compared with type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. In the condition of treating the cells with reductive GSH, proliferation was promoted in typeⅠendometrium cancer cells while it was inhibited in typeⅡendometrium cancer cells. The ROS level was decreased in both typeⅠand Ⅱ endometrium cancer cells. The GSH level, GSH/GSSG ratio, and SOD, CAT and GPx activities were reduced in type Ⅰ endometrium cancer cells while the GSH and GSSG levels, and CAT and GPx activities were decreased and the GSH/GSSG ratio was markedly increased in type Ⅱ endometrium cancer cells. ConclusionGlutathione definitely differently affects proliferation of typeⅠand Ⅱ endometrium cancer cells, which is related to the ROS level and activities of antioxidative systems in two type of endometrium cancer cells.

Key words: Endometrium neoplasms; Reactive oxygen species; Glut athione; Antioxidative systems

中图分类号:

R737.33

. 谷胱甘肽对KLE与HEC1b子宫内膜癌细胞生长的影响[J]. 山东大学学报(医学版), 2009, 47(9): 58-61.

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