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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (4): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.1097

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利拉鲁肽对脊髓损伤修复作用的探讨

李红志1,2,刘静3,宋岩1,2,迟令懿1,2,刘玉光1,2   

  1. 1.山东大学齐鲁医院神经外科, 山东 济南 250012;2.山东大学脑科学研究所, 山东 济南 250012;3.泰安市中心医院麻醉科, 山东 泰安 271000
  • 收稿日期:2015-11-26 出版日期:2016-04-10 发布日期:2016-04-10
  • 通讯作者: 迟令懿. E-mail:chilingyi@126.com刘玉光. E-mail:ns3000@126.com E-mail:chilingyi@126.com
  • 基金资助:
    山东省中青年科学家科研奖励基金(BS2010YY026);山东大学自主创新基金(2012TS144,2015TS017)

Role of liraglutide in the repair of spinal cord injury

LI Hongzhi1,2, LIU Jing3, SONG Yan1,2, CHI Lingyi1,2, LIU Yuguang1,2   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Brain Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Anesthesia, Taian Central Hospital, Taian 271000, Shandong, China
  • Received:2015-11-26 Online:2016-04-10 Published:2016-04-10

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摘要： 目的 探讨利拉鲁肽对大鼠脊髓损伤(SCI)神经的修复作用及机制。 方法 将雌性大鼠分为利拉鲁肽干预组、正常对照组,每组24只。采用大鼠脊髓动脉瘤夹夹伤法建立大鼠脊髓损伤模型。术后分别给予利拉鲁肽(0.12 mg/kg,0.5 mL)、生理盐水(0.5 mL)。术后2 d应用细胞凋亡技术检测神经细胞损伤程度,术后3 d采用 Western blotting蛋白印迹技术检测损伤脊髓组织中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达水平,术后14 d对损伤脊髓组织行HE染色观察组织形态变化并计算损伤空洞面积,术后3 d至28 d定期记录大鼠后肢行为学BBB评分。 结果 SCI后利拉鲁肽干预组细胞凋亡、炎性因子表达、空洞面积计算均较正常对照组降低(P<0.05),利拉鲁肽干预组双后肢BBB评分同期较正常对照组高(P<0.05)。 结论 脊髓损伤后给予利拉鲁肽可降低细胞凋亡及急性炎症反应,有利于后期神经组织修复以及运动功能恢复。

关键词: 脊髓损伤, 利拉鲁肽, 细胞凋亡, 白细胞介素-1β, 大鼠, 白细胞介素-6, 炎症

Abstract: Objective To investigate the role and mechanism of liraglutide in the repair of spinal cord injury(SCI)in rats. Methods A total of 48 rat models of clip compressive SCI were established and then divided into 2 groups: liraglutide treatment group(n=24)and normal control group(n=24), which were injected with liraglutide(0.12 mg/kg, 0.5 mL)and physiological saline(0.5 mL)respectively after the operation. The apoptosis of neurocytes was detected with TUNEL method on day 2 after operation, the expressions of interleukin-1β and interleukin-6 were detected with Western blotting protein imprinting technology on day 3, the structure of injured spinal cord tissue was observed with HE staining on day 14, and the BBB score of hind limbs of rats behavior were recorded from day 3 to day 28. Results The rate of cell apoptosis, expression of inflammatory factors, and cavity area of the liraglutide treatment group were lower than those of the control group(all P<0.05), while the BBB scores of both hind limbs were higher than those in the control group at the same period(P<0.05). Conclusion Liraglutide treatment after spinal cord injury may reduce cell apoptosis acute inflammation, and is conducive to the late repair of nerve cells and the recovery of motor function.

Key words: Spinal cord injury, Liraglutide, Cell apoptosis, Interleukin-1β, Interleukin- 6, Inflammation, Rats

中图分类号: 

  • R574
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