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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (5): 31-35.doi: 10.6040/j.issn.1671-7554.0.2015.047

• 基础医学 • 上一篇    下一篇

病毒性心肌炎小鼠调节性B细胞的变化

岑治宏, 郭予洁, 伍伟锋, 李丽萍, 周秋曦   

  1. 广西医科大学第一附属医院心内科, 广西 南宁 530021
  • 收稿日期:2015-01-13 修回日期:2015-04-01 出版日期:2015-05-10 发布日期:2015-05-10
  • 通讯作者: 伍伟锋。E-mail:wucna65@163.com E-mail:wucna65@163.com
  • 基金资助:
    国家自然科学基金(81450055)

Changes of regulatory B cells in mice with viral myocarditis induced by Coxsackie virus

CEN Zhihong, GUO Yujie, WU Weifeng, LI Liping, ZHOU Qiuxi   

  1. Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2015-01-13 Revised:2015-04-01 Online:2015-05-10 Published:2015-05-10

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摘要： 目的 观察病毒性心肌炎小鼠不同时间点调节性B淋巴细胞(Breg)亚群的变化,探讨其在病毒性心肌炎发病中的作用。方法 将小鼠随机分为实验组和对照组,实验组腹腔注射柯萨奇病毒B3(CVB3),对照组注射等量磷酸盐缓冲液(PBS)。依据注射后的时间,实验组和对照组均设1周和2周时间点亚组。应用苏木素伊红染色后观察心肌病理改变并计算心肌病理积分,采用RT-PCR法检测心肌组织中CVB3 mRNA的表达水平,采用流式细胞术检测小鼠脾脏Breg细胞亚群占CD19+细胞的比例。结果 实验组心肌炎症水平及CVB3 mRNA表达水平均明显高于对照组;实验组1周亚组心肌组织病理积分低于2周亚组,但实验组1周亚组心肌CVB3 mRNA表达水平却高于2周亚组,差异均有统计学意义(P<0.05)。实验组小鼠脾Breg细胞比例均高于对照组,且实验组1周亚组小鼠脾Breg细胞比例高于2周亚组,差异有统计学意义(P<0.05)。结论 病毒性心肌炎小鼠中调节性B细胞表达增高,其可能参与病毒性心肌炎的发病过程。

关键词: 肠道病毒属, CD19+CD1dhiCD5+ B细胞, 心肌炎, 炎症, 调节性B细胞

Abstract: Objective To observe the changes of regulatory B (Breg) cells in mice with viral myocarditis (VMC) induced by coxsackie virus B3 (CVB3), and to explore the role of Breg cells in VMC mice. Methods Balb/c male mice were randomly divided into experimental group and control group. Mice in the experimental group were intraperitoneally infected with CVB3 to establish VMC models, while mice in the control group were treated with phosphate-buffered saline (PBS) intraperitoneally. According to the time after injection of CVB3 or PBS, all mice in the two groups were randomly divided into 1-week and 2-week subgroups. Myocardial histopathological scores were determined in hematoxylin eosin stained sections. Expression of CVB3 mRNA in heart was measured with real-time reverse transcription-polymerase chain reaction (RT-PCR), and the frequency of splenic Breg subsets in CD19+ B cells was measured with flow cytometry. Results There were significant differences in the pathological scores and expression of myocardial CVB3 mRNA between the VMC mice and controls. The pathological scores of 1-week experimental subgroup were lower than those of 2-week experimental subgroup, while the expression of myocardial CVB3 mRNA in 1-week experimental subgroup was higher than that in 2-week experimental subgroup (all P<0.05). Compared with controls, the frequency of splenic Breg cells in the VMC mice increased significantly at different time points. Moreover, the frequency of splenic Breg cells in 1-week experimental subgroup was higher than that in the 2-week experimental subgroup (all P<0.05). Conclusion Breg cells increase in mice with CVB3-induced VMC, indicating that Breg cells mayplay an important role in the pathogenesis of CVB3-induced VMC.

Key words: Inflammation, Myocarditis, CD19+CD1dhiCD5+ B cells, Enterovirus, Regulatory B cells

中图分类号: 

  • R542.2
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