miR-584对人胶质母细胞瘤侵袭和迁移能力的影响

doi:10.6040/j.issn.1671-7554.0.2014.965

摘要/Abstract

摘要： 目的探讨miR-584对人胶质母细胞瘤U251细胞侵袭和迁移能力的影响.方法将化学合成的miR-584-3p在脂质体Lipofectamine 2000的介导下,瞬时转染人胶质母细胞瘤U251,细胞分为空白对照组(无转染,其他条件相同)、mimics NC组(转染miR-584-3p mimics阴性对照序列)、miR-584-3p mimics组(转染miR-584-3p mimics)、inhibitor NC组(转染miR-584-3p inhibitor阴性对照序列)和miR-584-3p inhibitor组(转染miR-584-3p inhibitor).Real-time PCR法检测细胞中miR-584-3p的表达水平;CCK-8法检测细胞增殖能力;划痕实验和Transwell小室实验检测细胞的迁移和侵袭能力.结果与空白对照组和mimics NC组相比,miR-584-3p mimics组的miR-584-3p表达上调约100倍(P <0.05), 细胞增殖能力无明显差异(P >0.05),细胞侵袭和迁移能力明显下降(P <0.05); 与空白对照组和inhibitor NC组相比,miR-584-3p inhibitor组的miR-584-3p表达下调至空白对照组的5%(P <0.05), 细胞增殖能力无明显差异(P >0.05),细胞侵袭和迁移能力显著增强(P <0.05).结论 miR-584-3p mimics转染抑制了U251细胞的侵袭和迁移能力; miR-584-3p inhibitor转染能增强U251细胞的侵袭和迁移能力.

关键词: 胶质母细胞瘤, miR-584, 迁移, 侵袭

Abstract: Objective To explore the effect of miR-584 expression on the invasion and migration of glioblastoma U251 cells in vitro. Methods Chemosynthetic miR-584-3p was transfected into glioblastoma cells under the induction of Lipofectamine 2000. The cells were divided into control group(no transfection), mimics NC group(transfected with negative control sequence of miR-584-3p mimics), miR-584-3p mimics group(transfected with miR-584-3p mimics), inhibitor NC group(transfected with negative control sequence of miR-584-3p inhibitor), miR-584-3p inhibitor group(transfected with miR-584-3p inhibitor).The expression of miR-584-3p was detected by Real-time PCR. Cell proliferation was detected by CCK-8 proliferation assay. The invasive and migratory effects of glioblastoma cell were measured by Wound healing and Transwell assays. Results Compared with mimics NC group and control group, the expression of miR-584-3p in miR-584-3p mimics group was upregulated by 100 times(P< 0.05). Compared with mimics NC group and control group, the cell proliferation ability of miR-584-3p mimics group showed no significant change(P >0.05), but the cellular migratory and invasive activity of miR-584-3p mimics group were down-regulated significantly(P <0.05). Compared with inhibitor NC group and control group, the expression of miR-584-3p in miR-584-3p inhibitor group was down-regulated by 20 times(P <0.05). Compared with inhibitor NC group and control group, the cell proliferation ability of miR-584-3p inhibitor group showed no significant change(P >0.05), the cellular migration and invasive activity in miR-584-3p inhibitor group were up-regulated significantly(P <0.05). Conclusion The migration and invasion of glioblastoma cells can be inhibited by the over-expression of miR-584-3p, while the transfection of miR-584-3p inhibitor can enhance the migration and invasion of glioblastoma cells.

Key words: MiR-584, Glioblastoma, Invasion, Migration

中图分类号:

R739.41

韩晓, 薛皓, 闫绍峰, 郭兴, 李彤, 郭小凡, 高校, 李刚. miR-584对人胶质母细胞瘤侵袭和迁移能力的影响[J]. 山东大学学报（医学版）, 2015, 53(4): 16-21.

HAN Xiao, XUE Hao, YAN Shaofeng, GUO Xing, LI Tong, GUO Xiaofan, GAO Xiao, LI Gang. Effects of miR-584 on the invasion and migration of human glioblastoma U251 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 16-21.

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