胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响

doi:10.6040/j.issn.1671-7554.0.2014.533

摘要/Abstract

摘要： 目的观察胰高血糖素样肽-1(GLP-1)对高脂饲养大鼠受损卵巢生物钟的治疗作用.方法 Wistar大鼠随机分为普通饲养组、高脂饲养组及高脂饲养+GLP-1干预组;8周后测空腹血糖、胰岛素、胆固醇、甘油三酯、雌二醇、卵泡刺激素、黄体生成素的水平;实时定量PCR检测卵巢相关基因及生物钟基因的表达.结果 GLP-1对高脂饲养大鼠血清生化指标产生一定影响.与普通饲养组相比,高脂饲养组大鼠始基卵泡募集因子抗苗勒氏管激素(AMH)、生长分化因子-9(GDF-9)的表达水平降低(P<0.05),而高脂饲养GLP-1干预组AMH、GDF-9的mRNA的表达明显改善.此外,与普通饲养组相比,高脂饲养组大鼠卵巢生物钟相关基因的表达具有明显变化,而GLP-1可改善高脂饲养对卵巢生物钟相关基因表达的影响.结论 GLP-1对高脂饲养所致的卵巢生物钟受损具有改善作用.

关键词: 大鼠, 高脂饲养, 生物钟, 卵巢, 胰高血糖素样肽-1

Abstract: Objective To explore the therapeutic effect of glucagon-like peptide-1(GLP-1) on impaired circadian gene of ovary in rats fed with high-fat diet. Methods Wistar rats were randomly divided into control group, high-fat diet group and GLP-1 intervention group. Eight weeks later, the levels of fasting glucose, insulin, cholesterol, triglyceride, E2, FSH and LH were tested. The expression of ovary related gene and circadian gene in ovary tissues were detected with Real-time PCR. Results GLP-1 had certain effect on serum biochemical indexes in rats fed with high fat. Real-time PCR results showed that, compared with control group, the levels of primordial follicle recruitment factor anti-mullerian homone (AMH) and growth differentiation factor (GDF-9) were significantly decreased in rats fed with high-fat diet (P<0.05). However, the expression of AMH and GDF-9 mRNA in high-fat diet rats treated with GLP-1 were markedly elevated. Besides, compared with control group, the expression of ovary circadian clock related genes was affected in rats fed with high-fat diet, and GLP-1 could improve the expression of ovary circadian gene. Conclusion GLP-1 could ameliorate the expression of ovary circadian gene in rats fed with high-fat diet.

Key words: Circadian clock, High-fat feeding, Glucagon-like peptide-1, Ovary, Rats

中图分类号:

R711.6

姚志娜, 韩婷, 范秀玲, 章由生, 宋佳伦. 胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响[J]. 山东大学学报（医学版）, 2015, 53(2): 19-21.

YAO Zhina, HAN Ting, FAN Xiuling, ZHANG Yousheng, SONG Jialun. Effect of Glucagon-like peptide-1 on ovarian circadian clock rhythm of rats fed with high fat diet[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 19-21.

参考文献

[1] Summa KC, Vitaterna MH, Turek FW. Environmental perturbation of the circadian clock disrupts pregnancy in the mouse[J]. PLoS One, 2012, 7(5): e37668. doi: 10.1371/journal.pone.0037668. Epub 2012 May 23.
[2] Alvarez JD, Hansen A, Ord T, et al. The circadian clock protein BMAL1 is necessary for fertility and proper testosterone production in mice[J]. J Biol Rhythms, 2008, 23(1): 26-36.
[3] Oike H, Oishi K, Kobori M. Nutrients, Clock Genes, and Chrononutrition[J]. Curr Nutr Rep, 2014, 3:204-212. eCollection 2014.
[4] Brown SA. Circadian clock-mediated control of stem cell division and differentiation: beyond night and day[J]. Development, 2014,141(16): 3105-3111.
[5] Ratajczak CK, Boehle KL, Muglia LJ. Impaired steroidogenesis and implantation failure in Bmal1-/-mice[J]. Endocrinology, 2009, 150(4): 1879-1885.
[6] Sellix MT, Menaker M. Circadian clocks in the ovary[J]. Trends Endocrinol Metab, 2010, 21(10):628-636.
[7] Nakamura TJ, Moriya T, Inoue S, et al. Estrogen differentially regulates expression of Per1 and Per2 genes between central and peripheral clocks and between reproductive and nonreproductive tissues in female rats[J]. J Neurosci Res, 2005, 82(5): 622-630.
[8] 洪天配. 新型口服降糖药DPP-4抑制剂: 从胰岛α细胞看2型糖尿病治疗的新进展[J]. 中国糖尿病杂志, 2012, 20(7): 558-560. HONG Tianpei. New anti-diabetic agents Dpp-4 inhibitors: Viewing the advance of treatment for T2DM from pancreatic α cells[J]. Chinese Journal Diabetes, 2012, 20(7): 558-560.
[9] 张秀荣, 徐继马, 严庆涛, 等. DJB术对2型糖尿病大鼠肠神经GLP-1受体表达影响的研究[J]. 山东大学学报:医学版, 2012, 50(11): 1-5. ZHANG Xiurong, XU Jima, YAN Qingtao, et al. Effects of duodenal jejunal bypass on the expression of GLP-1R in the enteric nervous system of type 2 diabetic rats[J]. Journal of Shandong University: Health Science, 2012, 50(11): 1-5.
[10] Ruby RJ, Armato JP, Pyke C, et al. GLP-1 Provoked Severe Hypoglycemia in an Individual With Type 2 Diabetes and a Benign Insulinoma[J]. Diabetes Care, 2014, 37(8): e177-178.
[11] Wang T, Gou Z, Wang F, et al. Comparison of GLP-1 Analogues versus Sitagliptin in the Management of Type 2 Diabetes: Systematic Review and Meta-Analysis of Head-to-Head Studies[J]. PLoS One, 2014, 9(8): e103798. doi: 10.1371/journal.pone.0103798. eCollection 2014.
[12] Kelly AC, Steyn LV, Kitzmann JP, et al. Function and expression of sulfonylurea, adrenergic, and glucagon-like peptide 1 receptors in isolated porcine islets[J]. Xenotransplantation, 2014, 21(4): 385-391.
[13] Tashiro Y, Sato K, Watanabe T, et al. A glucagon-like peptide-1 analog liraglutide suppresses macrophage foam cell formation and atherosclerosis[J]. Peptides, 2014, 54: 19-26.
[14] Wu Z, Liu S, Nair I, et al. (64)Cu labeled sarcophagine exendin-4 for microPET imaging of glucagon like peptide-1 receptor expression[J]. Theranostics, 2014, 4(8): 770-777.
[15] Salehi M, D'Alessio DA. Effects of glucagon like peptide-1 to mediate glycemic effects of weight loss surgery[J]. Rev Endocr Metab Disord, 2014, 15(3): 171-179.

相关文章 15

[1]	吴逸南葛志明李方贺红姜虹张运. 自发性高血压大鼠肾脏血管紧张素转换酶2的表达[J]. 山东大学学报(医学版), 2209, 47(6): 5-.
[2]	王伟王沂峰张岭梅林琼燕黄菊. 人卵巢癌OVCAR3细胞系中侧群细胞的分离及其成瘤性、侵袭性的实验研究[J]. 山东大学学报(医学版), 2209, 47(6): 8-11.
[3]	祝林胡三元张光永丁祥就. 前列腺素E2对阻塞性黄疸大鼠小肠粘膜形态的保护作用[J]. 山东大学学报(医学版), 2209, 47(6): 12-.
[4]	孙涛张道来谢珊珊王玉卓冯玉新辛华. 酒精对原代培养的神经前体细胞间隙连接蛋白43表达的影响[J]. 山东大学学报(医学版), 2209, 47(6): 20-.
[5]	张道来孙涛谢珊珊王玉卓赵玲冯玉新辛华. 体外原代培养胎鼠大脑皮层神经元NMDAR1亚基表达的发育性变化[J]. 山东大学学报(医学版), 2209, 47(6): 28-32.
[6]	黄玉真,吕琛,颜磊,李帅帅,张景雪,刘超,王银霞,桑岩岩,房鹛. 基于危险因素筛查的延续性院外管理对卵巢过度刺激综合征的预防效果[J]. 山东大学学报 (医学版), 2026, 64(4): 92-99.
[7]	万文静,高姗姗. 低分子肝素在生殖领域的应用[J]. 山东大学学报 (医学版), 2025, 63(6): 117-124.
[8]	袁宗怀,潘广晔,迟曰梅,安传国,张永刚. 孟德尔随机化分析低级别浆液性卵巢癌与乳腺癌的因果关系[J]. 山东大学学报 (医学版), 2025, 63(1): 99-107.
[9]	潘静,于潇,刘金星,刘鹏飞. 始基卵泡激活调控的研究进展[J]. 山东大学学报 (医学版), 2024, 62(8): 74-92.
[10]	赵子辉,王旭阳,张鹏,方宁宁,石端博,杨帆,杨晓玫,吴剑波. 经膈肌穿刺法快速准确评估大鼠肺动脉高压[J]. 山东大学学报 (医学版), 2024, 62(2): 20-28.
[11]	刘昶,王倩男,张悦,陈忠绍,褚然,姚舒. 卵巢交界性肿瘤保留生育功能术后肿瘤预后及妊娠结局[J]. 山东大学学报 (医学版), 2023, 61(8): 61-66.
[12]	祁少俊,唐延金,张正铎,吴虹,张佳程,秦川,刘锐,高希宝. 补充多种微量元素对高糖饮食大鼠的保护作用[J]. 山东大学学报 (医学版), 2023, 61(7): 19-26.
[13]	吴磊丽,蔡华丽,孙德胜,魏蔚霞. 超声引导穿刺硬化术联合地诺孕素治疗卵巢子宫内膜异位囊肿的疗效[J]. 山东大学学报 (医学版), 2023, 61(6): 65-69.
[14]	何静,严如根,武志红,李长忠. 消癥抑癌方对卵巢癌SKOV3细胞增殖、迁移的影响[J]. 山东大学学报 (医学版), 2023, 61(5): 1-10.
[15]	赵元元,路军涛,吴小华. 人脐带间充质干细胞外泌体miR-100对多囊卵巢综合征患者颗粒细胞炎症的影响[J]. 山东大学学报 (医学版), 2023, 61(5): 51-58.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed