酸浆苦素B对人非小细胞肺癌细胞增殖、迁移及凋亡的影响

doi:10.6040/j.issn.1671-7554.0.2018.661

摘要/Abstract

摘要： 目的探讨酸浆苦素B(PB)对非小细胞肺癌(NSCLC)A549细胞增殖、迁移和凋亡的影响,初步探讨其作用机制。方法细胞分为不同浓度(2.5、5、10、20、40 μmol/L)PB组、对照组、空白组,实时无标记细胞分析技术(RTCA)、CCK-8法检测PB对A549细胞增殖抑制率变化,Transwell检测细胞迁移,流式细胞仪检测PB对A549细胞的凋亡及周期的影响,Western blotting检测PB对相关蛋白表达的影响。结果 2.5、5、10、20、40 μmol/L PB可抑制A549细胞增殖,且呈剂量依赖性;Transwell结果显示,10、20 μmol/L PB组较对照组的细胞迁移能力下降;10、20 μmol/L PB处理后,A549细胞的凋亡率升高,呈剂量依赖性。与对照组相比,10、20 μmol/L PB组G₂/M期细胞比例增高、G₀/G₁期比例减少。Western blotting检测结果显示,与对照组相比,10、20 μmol/L PB组使Akt蛋白表达水平下降,而Caspase-9和PTEN蛋白表达水平上升。结论 PB可抑制A549细胞的增殖,诱导细胞凋亡且细胞周期阻滞,这可能与调控细胞周期相关蛋白表达有关。

关键词: 非小细胞肺癌, 酸浆苦素B, 增殖, 凋亡, 迁移

Abstract: Objective To investigate the effects of Physalin B(PB)on proliferation, migration and apoptosis of A549 cells in non-small cell lung cancer(NSCLC), and preliminarily explore its mechanism. Methods The A549 cells were divided into different concentrations(2.5, 5, 10, 20, 40 μmol/L)of PB groups, control group and blank group. The inhibition rate of PB on A549 cells proliferation was detected by real-time cell analyzer(RTCA)and CCK-8 assay, cell migration was examined by transwell chamber assay, the apoptosis and cycle of PB on A549 cells were detected by flow cytometry and the effect of PB on the expression of related proteins was detected by Western blotting. Results PB with 2.5, 5, 10, 20 and 40 μmol/L significantly inhibited the proliferation of A549 cells in a dose-dependent manner. The cell migration ability of 10 and 20 μmol/L PB groups was significantly lower than that of control group. After treatment with 10 and 20 μmol/L PB, the apoptosis rate of A549 cells increased in a dose-dependent manner. Compared with control group, the proportion of G₂/M phase cells increased and the proportion of G₀/G₁ phase cells decreased in 10 and 20 μmol/L PB groups. Compared with the control group, the expression level of Akt protein decreased while the expression levels of Caspase-9 and PTEN increased in 10 and 20 μmol/L PB groups. Conclusion PB can inhibit the proliferation of A549 cells, induce cell apoptosis and cell cycle arrest, which may be related to the regulation of cell cycle related protein expression.

Key words: Non-small cell lung cancer, Physalin B, Proliferation, Apoptosis, Transfer

中图分类号:

R734.2

王伟,刘拥征,李岭. 酸浆苦素B对人非小细胞肺癌细胞增殖、迁移及凋亡的影响[J]. 山东大学学报 (医学版), 2019, 57(3): 13-18.

WANG Wei, LIU Yongzheng, LI Ling. Effects of Physalin B on proliferation, migration and apoptosis of human non-small cell lung cancer cells[J]. Journal of Shandong University (Health Sciences), 2019, 57(3): 13-18.

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