关键词: 骨肉瘤, 细胞凋亡, 大黄素, ERK1/2

Abstract: Objective  To explore the inhibitory effect of emodin on the proliferation of human osteosarcoma cells SOSP9607 and the related mechanisms. Methods  Human osteosarcoma cells SOSP9607 were divided randomly into control group and treatment groups (emodin: 25, 50 and 100μmol/L). The growth inhibition ratio (IR) of emodin on osteosarcoma cells was detetced with MTT assay. The apoptosis was analyzed with flow cytometry. The changes of expressions of caspase-3, caspase-9, ERK1/2 and p-ERK1/2 were tested with Western blotting. Results  All of the cells in the treatment groups were obviously inhibited compared to those in control group, which was dose-dependent. Compared with control group, the apoptosis ratio of treatment group increased. Western blotting showed that the expressions of caspase-3 and caspase-9 protein increased in treatment group compared to control group, while the expression of p-ERK1/2 protein decreased. Conclusion  Emodin could inhibit the proliferation of human osteosarcoma cell by suppressing ERK signaling pathway and activating caspase-3 and caspase-9.

Key words: Emodin, ERK1/2, Apoptosis, Osteosarcoma