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山东大学学报(医学版) ›› 2209, Vol. 47 ›› Issue (6): 99-102.

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多药耐药基因1 C1236T多态性与癫痫患者对药物反应性的相关性

葛丽娟 金瑞峰 王纪文 许新升 李癊   

  1. 1. 山东大学齐鲁医院儿科, 济南 250012; 2. 山东大学齐鲁儿童医院儿科, 济南 250022;
    3. 淄博红十字会昌国医院, 山东 淄博 255000
  • 收稿日期:2009-02-18 出版日期:2009-06-16 发布日期:2209-06-25
  • 通讯作者: 王纪文(1966- ),男,教授,博士,主要研究方向为小儿神经系统疾病。 E-mail:wangjiwen-99@hotmail.com
  • 作者简介:葛丽娟(1983- ),女,硕士研究生,主要研究方向为癫痫耐药机制。
  • 基金资助:

    济南市科技攻关项目(2007050951)。

Association between the C1236T polymorphism in multi-drug resistance gene 1 and response to antiepileptic drug treatment in epileptic patients

 GE Li-Juan, JIN Rui-Feng, WANG Ji-Wen, HU Xin-Sheng, LIKun   

  1. 1. Department of Pediatrics,Qilu Hospital of Shandong University, Jinan 250012, China; 2. Department of Pediatrics,Qilu Children's Hospital of Shandong University, Jinan 250022, China; 3. Zibo Changguo Hospital of Red Cross Association, Zibo 255000, Shandong, China
  • Received:2009-02-18 Online:2009-06-16 Published:2209-06-25

摘要:

目的 了解多药耐药基因1(MDR1) C1236T多态性在癫痫患者中的分布,并探讨其与癫痫患者耐药的相关性。方法 选择70例难治性癫痫患者及88例对抗癫痫药物敏感的癫痫患者,提取其外周血DNA,应用多聚合酶链反应(PCR)扩增后继以限制性内切酶片断长度多态性(RFLP)分析,检测基因MDR1的第12个外显子中C1236T的多态性。结果 两组病例的基因型频率和等位基因频率相比较,差异均无统计学意义(P>0.05)。结论 MDR1C1236T多态性与癫痫患者对抗癫痫药物反应没有相关性。

关键词: 癫痫;多药耐药基因;单核苷酸多态性;关联反应

Abstract:

Objective To determine the frequency of polymorphism at C1236T of multidrug resistance 1 gene (MDR1) in epileptic patients and explore the association of this polymorphism with antiepileptic drug treatment. Methods  DNA samples were obtained from 158 patients, of whom 70 were resistant to antiepileptic drug treatment and 88 were responsive to the treatment. Genotypes of the C1236T polymorphism were determined by polymerase chain reaction(PCR)followed by restriction fragment length polymorphism (RFLP). Allele frequencies in the drug resistant group were compared with those in the response group by Chisquare analysis. Results Of 158 patients, 30(19.4% )had the CC genotype, 49(30.6%)had the CT genotype,and 79(50.0% )had the TT genotype. Frequency of CC genotype was significantly higher in the pharmacoresistance group(22.9% )than in the responsive group(15.9%), and frequency of the C allele was also higher in the pharmacoresistance group (36.4%) than in the responsive group(33.0%). But there were no significant differences in both genotype frequency and allele frequency between the two groups. Conclusion There is no association between the locus of C1236T in the MDRI gene and response to antiepileptic drug treatment in Chinese patients with epilepsy.

Key words: Epilepsy; Multidrug resistance gene; Polymorphism; Association

中图分类号: 

  • R742.1
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[13] 葛丽娟 金瑞峰 王纪文 许新升 李癊. 多药耐药基因1 C1236T多态性与癫痫患者对药物反应性的相关性[J]. 山东大学学报(医学版), 2009, 47(6): 99-102.
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