DDR2在Peroxynitrite诱导人中枢血管平滑肌细胞凋亡中的表达

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (6): 9-12.

DDR2在Peroxynitrite诱导人中枢血管平滑肌细胞凋亡中的表达

李林1，陈晓锐2，宋莎莎1，焦玉莲1，马春燕1，鞠远荣3，李建峰1

1.山东大学附属省立医院中心实验室, 济南 250021； 2.滨州医学院医学人文学院，山东烟台 264003；
3.山东大学附属省立医院ICU室，济南 250021

收稿日期:2010-01-03 出版日期:2010-06-16 发布日期:2010-06-16
通讯作者: 李建峰（1962- ）,男, 教授, 博士生导师,主要从事血管生物学的研究工作。 E-mail: lijianfeng@hotmail.com
作者简介:李林（1981- ）,男, 硕士研究生, 主要从事肿瘤病理学和血管生物学的研究工作。 E-mail: gestapo0903@sina.com
基金资助:
山东省科技厅基金资助课题（2006GG2202）。

Expression of DDR2 in human cerebral vascular smooth muscle cells apoptosis subjected to peroxynitrite

LI Lin1， CHEN Xiaorui2， SONG Shasha1， JIAO Yulian1, MA Chunyan1, JU Yuanrong3, LI Jianfeng1

1. Central Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
2. Medical College of Binzhou, Yantai 264003, Shandong, China;
3. ICU, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2010-01-03 Online:2010-06-16 Published:2010-06-16

摘要/Abstract

摘要：

目的探究过氧亚硝酸盐(peroxynitrite, ONOO-) 诱导人中枢血管平滑肌细胞（HCVSMCs）凋亡过程中盘状结构域受体 (DDR2)基因表达的变化。方法以不同浓度的ONOO-作用于体外培养的HCVSMCs，采用噻唑蓝(MTT)比色法测定细胞生存率，流式细胞仪测定细胞凋亡率，吖啶橙染色进行形态学观察。同时, 采用逆转录-聚合酶链反应(RT-PCR)及Western blot 检测DDR2 mRNA及蛋白表达。结果与对照组相比，以ONOO-作用于HCVSMCs 24h可显著抑制该系细胞增生,诱导细胞凋亡(P<0.05)，并引起DDR2 mRNA及蛋白水平总体呈现下调趋势(P<0.05)。结论 DDR2基因可能在ONOO-诱导HCVSMCs凋亡的信号转导调节通路中起关键作用。

关键词: 盘状结构域受体2；人中枢血管平滑肌细胞；过氧亚硝酸盐；细胞凋亡

Abstract:

Objective To investigate the expression of DDR2 gene in the human cerebral vascular smooth muscle cells (HCVSMCs) exposed to peroxynitrite (ONOO-). Methods HCVSMCs cultured in vitro were subjected to different concentrations of ONOO-. Cell viability was measured by MTT assay. The rate of cell apoptosis was measured by flow cytometer. The morphological changes were assessed by acridine orange staining to determine whether cell experienced apoptosis under the stress of ONOO-. The expression of DDR2 gene at both mRNA and protein levels was examined by the means of RT-PCR and Western-blot simultaneously. Results Direct exposure of cells to ONOO- was able to block the cell proliferation in a concentration-dependent manner via the apoptotic pathway. Under such a stress, the expression of DDR2 gene was finally decreased at both mRNA and protein levels. Conclusion DDR2 gene may play a pivotal role in ONOO-induced apoptosis in HCVSMCs.

Key words: DDR2 gene; Human cerebral vessel smooth muscle cell; Peroxynitrite; Apoptosis

中图分类号:

R362

李林1，陈晓锐2，宋莎莎1，焦玉莲1，马春燕1，鞠远荣3，李建峰1. DDR2在Peroxynitrite诱导人中枢血管平滑肌细胞凋亡中的表达[J]. 山东大学学报(医学版), 2010, 48(6): 9-12.

LI Lin1， CHEN Xiaorui2， SONG Shasha1， JIAO Yulian1, MA Chunyan1, JU Yuanrong3, LI Jianfeng1. Expression of DDR2 in human cerebral vascular smooth muscle cells apoptosis subjected to peroxynitrite[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 9-12.

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