CPI-17调控血管平滑肌表型对动脉粥样硬化形成的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (6): 13-.

CPI-17调控血管平滑肌表型对动脉粥样硬化形成的影响

席文锦1，王艳3，娄运伟1，张佳慧2,梁拓2，章桂忠1，张然熙2，刘素侠1

山东大学 1.医学院免疫学研究所, 济南 250012； 2.医学院济南 250012；
3.齐鲁医院心内科, 济南 250012

收稿日期:2010-01-16 出版日期:2010-06-16 发布日期:2010-06-16
通讯作者: 刘素侠（1972- ），女，副教授，主要从事分子免疫研究。 E-mail:liusuxia1972@sdu.edu.cn
作者简介:席文锦（1983- ），女，硕士研究生，主要从事分子免疫研究。
基金资助:
山东省自然科学基金资助课题（Z2006C04）。

CPI-17 affects atherosclerosis by regulating the phenotype of vascular smooth muscle cells

XI Wenjin1, WANG Yan3, LOU Yunwei1, ZHANG Jiahui2, LIANG Tuo2, ZHANG Guizhong1, ZHANG Ranxi2, LIU Suxia1

Shandong University 1. Institute of Immunology, School of Medicine, Jinan 250012, China;
2. School of Medicine, Jinan 250012, China; 3. Department of Cardiology, Qilu Hospital, Jinan 250012, China

Received:2010-01-16 Online:2010-06-16 Published:2010-06-16

摘要/Abstract

摘要：

目的研究CPI-17（PKC-potentiated protein phosphatase inhibitory protein-17）通过诱导血管平滑肌细胞表型改变影响动脉粥样硬化形成的作用。方法载脂蛋白E基因敲除（ApoE-knockout）小鼠经颈动脉套管术诱导形成动脉粥样斑块，以野生型C57BL/6小鼠为对照，分离颈动脉并提取总RNA，采用实时荧光定量PCR技术对比检测斑块组织与对照小鼠颈动脉中CPI-17及血管平滑肌细胞收缩功能相关蛋白-平滑肌肌球蛋白重链（sm-MHC）、α-肌动蛋白（α-actin）的mRNA水平。然后分别用50mg/L氧化型低密度脂蛋白（ox-LDL）刺激及乏氧环境下培养作用于小鼠平滑肌细胞系MOVAS 细胞，实时荧光定量PCR检测两种刺激条件下CPI-17表达的变化。结果 ApoE-knock out小鼠粥样斑块平滑肌细胞的CPI-17 mRNA水平及sm-MHC、α-actin显著低于C57BL/6小鼠，ox-LDL刺激及乏氧培养的MOVAS细胞的CPI-17表达水平显著降低（P均<0.05）。结论 ox-LDL刺激及乏氧培养使MOVAS 细胞的CPI-17表达水平降低，可能通过调控血管平滑肌细胞的收缩特性参与动脉粥样硬化的发生。

关键词: 基因，CPI-17；血管平滑肌细胞；动脉硬化；实时荧光定量多聚酶链反应

Abstract:

Objective To investigate the role of CPI-17 in atherosclerosis by inducing the phenotype changing in vascular smooth muscle cells. Methods We established an atherosclerotic plaque model using ApoE=knockout mice, separated the carotid artery and extracted the total RNA, detected the level of CPI-17 mRNA in both atherosclerotic plaque and normal artery of C57BL/6 mice. Smooth muscle α-actin and sm-MHC were also detected. Furthermore, MOVAS cells were stimulated separately using two methods: ox-LDL at 50mg/L and cultured under hypoxia. The expression of CPI-17 was determined in these cells. Results The level of CPI17 mRNA in atherosclerotic plaque of ApoE-knockout mice was significantly lower than that in C57BL/6 mice. The expression of smooth muscle α-actin and smooth muscle myosin heavy chain(sm-MHC) were down-regulated in atherosclerotic plaque in comparison with normal artery. The level of CPI-17 mRNA was down-regulated in MOVAS cells stimulated with ox-LDL and cultured under hypoxia. Conclusion CPI-17 may play important role during atherosclerosis by inducing phenotype changing of vascular smooth muscle cells.

Key words: Gene, CPI-17; Vascular smooth muscle cell; Atherosclerosis; Quantitative polymerase chain reaction

中图分类号:

Q344.14

席文锦1，王艳3，娄运伟1，张佳慧2,梁拓2，章桂忠1，张然熙2，刘素侠1. CPI-17调控血管平滑肌表型对动脉粥样硬化形成的影响[J]. 山东大学学报(医学版), 2010, 48(6): 13-.

XI Wenjin1, WANG Yan3, LOU Yunwei1, ZHANG Jiahui2, LIANG Tuo2, ZHANG Guizhong1, ZHANG Ranxi2, LIU Suxia1. CPI-17 affects atherosclerosis by regulating the phenotype of vascular smooth muscle cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 13-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed