单核细胞被诱导表达淋巴管内皮标志物

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 85-88.

单核细胞被诱导表达淋巴管内皮标志物

王长明¹,田铧¹ ,梁艳红¹,于伟² ,肖琼¹,张肇林¹

1. 山东大学医学院解剖与组织胚胎学研究所，济南 250012； 2. 章丘市人民医院急救中心，山东章丘 250200

收稿日期:2009-03-03 发布日期:2009-11-16
通讯作者: 田铧（1958- ），女，教授，硕士生导师，主要从事淋巴管再生与免疫研究。 Email：sduth@163.com
作者简介:王长明（1982- ），男，硕士研究生，主要从事淋巴管再生研究。
基金资助:
山东省自然科学基金项目（Y2005C14）。

Monocytes were induced to express the markers of lymphatic endothelial cells

WANG Changming ¹, TIAN Hua ¹, LIANG Yanhong ¹ , YU Wei² , XIAO Qiong ¹, ZHANG Zhaolin ¹

1. Institute of Anatomy & Histology and Embryology, Medical School of Shandong University, Jinan 250012, China;
2. The People′s Hospital of Zhangqiu City, Zhangqiu 250200, Shandong, China

Received:2009-03-03 Published:2009-11-16

摘要/Abstract

摘要：

目的淋巴管生成存在于肿瘤、炎症等许多疾病的病理生理过程中。本研究旨在探讨单核-巨噬细胞在炎症环境中转分化成淋巴管内皮细胞的可能性。方法取成人新鲜外周血，分离单核细胞，用ECM培养，分别在FN铺板或VEGFC、TNFα、LPS刺激下诱导培养24?h，用RTPCR和免疫荧光细胞化学方法检测单核-巨噬细胞对淋巴管内皮特异性标志物LYVE1、Podoplanin、Prox1及内皮细胞共同标志物vWF、eNOS的基因和蛋白表达。结果在未诱导组，单核-巨噬细胞LYVE1呈阳性表达，Podoplanin、Prox1、vWF、eNOS表达呈阴性；在FN铺板或VEGFC、TNFα、LPS诱导组，单核-巨噬细胞Podoplanin、Prox1表达阳性，vWF和eNOS表达仍呈阴性。结论FN、VEGFC、TNFα、LPS均能有效刺激单核-巨噬细胞表达淋巴管内皮标志物。

关键词: 单核细胞；转分化；淋巴管内皮细胞；体外培养

Abstract:

Lymphangiogenesis is thought to be involved in the pathophysiological process such as tumors and inflammation. This research looked into the possibility of the transdifferentiation of mononuclearmacrophages into lymphatic endothelial cells in inflammatory conditions. MethodsFresh monocytes from peripheral blood were collected and cultured in ECM. The cells were next incubated in FNplated wells or treated with VEGFC, TNFα and LPS for 24 hours respectively. Expression of specific markers of lymphatic endothelial cells such as LYVE1, Podoplanin and Prox1, and the common markers of endothelial cells such as vWF and eNOS were detected by RTPCR and immunochemical methods. ResultsBefore the induction, expression of LYVE1 was positively detected in cultured mononuclearmacrophages, while the expression of vWF, eNOS, Podoplanin, Prox1 was negatively detected. After the induction, the level of expression of Podoplanin and Prox1 obviously increased in cultured mononuclearmacrophages, yet expression of vWF and eNOS remained negative. ConclusionMononuclearmacrophages can be induced to express the markers of lymphatic endothelial cells by FN, VEGFC, TNFα and LPS.

Key words: Monocytes; Transdifferentiation; Lymphatic Endothelial Cells; In Vitro

中图分类号:

R392.1

王长明1,田铧1,梁艳红1,于伟2,肖琼1,张肇林1. 单核细胞被诱导表达淋巴管内皮标志物[J]. 山东大学学报(医学版), 2009, 47(11): 85-88.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	曾兆清1，2，杜文军2，史兆章2，徐伟3，刘倩雯3，陈士俊4. 白细胞介素-17对肝星状细胞增殖及细胞周期的影响[J]. 山东大学学报(医学版), 2011, 49(4): 21-24.
[2]	韩建军1，胡三元1，智绪亭1，梁晓红2，薛德文3. 真核双基因表达载体pIRES-IL-24-ES的构建与鉴定[J]. 山东大学学报(医学版), 2010, 48(3): 78-82.
[3]	王灿，苏良，沈菲，陈士俊. 不同亚型的CD4+T淋巴细胞在干扰素抗乙肝病毒治疗中的作用[J]. 山东大学学报(医学版), 2010, 48(3): 109-112.