JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

In vivo vaccine effect and tumorigenicity of human breast carcinoma cells transfered by interleukin-18 gene

HAN Ming-yong1，LIU Qi1，TANG Bu-jian2，DENG Yan2，CAO Ming-feng1   

  1. 1. Department of Tumor Treatment Center, Shandong Provincial Hospital, Jinan 250021,Shandong, China; 2 Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2006-03-29 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
  • Contact: LIU Qi

PDF (PC)

523

Abstract: Objective: To study the effect of the interleukin-18 gene transfection on tumorigenesis of the human breast carcinoma cell line Bcap37. Methods: Bcap37 cells were transfected with a mammalin expression vector containing human IL-18 complementary DNA. The biological expression of IL-18 was determined by the RT-PCR and ELISA methods. Nude mice were injected with Bcap37 cells with or without the IL-18 gene. Results: The IL-18 cDNA was successfully integrated into the Bcap37 cells. There were (113.8±4.7)pg IL-18 secreted by one million transduced cells in 24 hours. In vivo tumor growth curves, based on measured tumor size, Bcap37, Bcap37-pcDNA3.1 and Bcap37-IL-18 resulted in tumor formation from 8 to 11 days after injection. The growth rate of Bcap37-IL-18 in vivo was significantly slower than that of Bcap37 and Bcap37-pcDNA3.1 cells. Tumor formation by parental Bcap37 cells was significantly delayed in mice that had been immunized with Bcap37-IL18 irradiated cells. Conclusion: Human breast carcinoma cells were successfully modified by IL-18 cytokine genes and reduced the tumorigenicity. Breast cells transduced with IL-18 genes might be used as human colon cancer vaccines.

Key words: Interleukin-18, Breast neoplasms, Transfection, Tumor cells, cultured, Vaccine

CLC Number: 

  • R739.9
[1] SUN Tao, ZHANG Dao-Lai, XIE Shan-Shan, WANG Yu-Zhuo, FENG Yu-Xin, XIN Hua. Effect of ethanol on expression of connexin 43 in primary culture of neural precursor cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 20-.
[2] ZHAO Hanqing, ZHOU Xinrui, LI Zijian, TANG Xing. Application of circulating tumor cells combined with serological detection in non-small cell lung cancer [J]. Journal of Shandong University (Health Sciences), 2025, 63(5): 79-85.
[3] GAO Lihe, REN Jingjing, LI Yan, LI Qiang, MA Wanshan, LI Huanjie, CHEN Zhen,OU Lanxiang, ZHANG Shaoming, ZHU Zhiwei, DING Xinglong, LI Hongxia, WANG Yan, ZHANG Zhongfa, WANG Yunshan. Preparation and application of an enzyme-linked immunoassay kit for SARS-CoV-2 neutralizing antibody [J]. Journal of Shandong University (Health Sciences), 2023, 61(4): 77-85.
[4] QIN Chaoqun, HUANG Bin, YANG Fang, WANG Changming, XIAO Ying, HUANG Hancan, LI Liying, GAO Feng. GSK3β/eEF2K signaling involves in the induced differentiation of lung fibroblasts by regulating autophagy [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 8-15.
[5] ZOU Liping, HE Yujing, XIE Yuanzhong, ZHAO Jianyun, XU Chunhua, MA Yongsheng, LI Chuanbin, LIU Xiaoxue, LIU Chong, ZHAO Xiaodong, SUN Hongyun. Time distribution and influencing factors of hepatitis B vaccine birth dose in 1,614 newborns of HBsAg positive women, Jinan City [J]. Journal of Shandong University (Health Sciences), 2022, 60(11): 113-120.
[6] Yuelong SHU,Simin WEN. Research progress on factors influencing the protective effects of influenza vaccines [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 1-7.
[7] Kai WANG. Current status and prospect of viral vaccines research and development [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 8-14.
[8] JIAO Fengping, ZHANG Haitao, ZHAO Yinghui, TIAN Zhaoju, YANG Shulin. Preliminary study of tissue distribution and safety of HSV-2 DNA vaccine in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 38-42.
[9] JIAO Fengping, WANG Yu, YU Ailian, TIAN Zhaoju, YANG Shulin. Immunogenicity of the eukaryotic expression vector pcDNA3.1(-)-P6-gBCTL-TBK-1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 75-78.
[10] CHEN Yikun, WANG Ketao, WANG Huayang, SHAO Qianqian, TAN Wanye, SONG Xiaobin, QU Xun, WEI Fengcai. Regulatory role of KIF4A in the expression of angiogenesis- associated factors of macrophages [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 39-43.
[11] WANG Lin, LÜ Gang, HAN Yali, GUO Jingjing, ZHAO Qunli, HE Shenyi. Construction of an eukaryotic vector of rhoptry protein 7 from Toxoplasma gondii and its immunoprotective effect [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 11-15.
[12] GAO Feng, KANG Bai, YANG Hongming, ZHAO Tingkun, QU Meihua, WANG Jinhong. Expression of the cutaneous T-cell lymphoma-associated antigen 5 in tumor cell lines [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 41-45.
[13] LIU Chengxia, LI Ming, LIAN Haifeng, SHI Ning. Impact of miR-486-5p on the biological behavior of colorectal carcinoma SW620 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 16-20.
[14] FU Haiyan, HU Zhansheng, DU Hongyang, LI Chao, BAO Cuifen. Effect of rehmannia glutinosa polysaccharide on the differentiation and proliferation of over-expressed Notch1(NICD) gene in rat bone marrow mesenchymal stem cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 34-40.
[15] ZHENG Jiatian, ZHU Kai, SUN Hongsheng, FU Min, PAN Zhenglun. Association of IL-18 and MCP-1 gene polymorphisms with rheumatoid arthritis: a meta-analysis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 98-104.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd