Abstract: Objective: To study the anti-calcification function property of rabbit thoracic aorta cross-linked by dye-mediated photo-oxidation. Methods: Rabbits whose allogenic thoracic aorta was cross-linked by dye-mediated photo-oxidation (DMP, n=6) were in the DMP group, rabbits whose allogenic thoracic aorta was cross-linked by glutaraldehyde (GA, n=6) were in the GA group, and rabbits whose homogenic abdominal aorta was orthotropically transplanted (OT, n=6) were in the OT group. Then the donor arteries were transplanted in the position of abdominal arteries of rabbits. After operations, animals were fed for 1 month, and then the grafted artery was taken off for observation by HE, SEM and tissue calcium content which was analyzed by flame atomic absorption spectrophotometer. Results: Results of calcium content in the DMP group, the GA group and the OT group was 3.58±0.22μg/mg, 22.5±0.53μg/mg and 2.99±0.33μg/mg, respectively. There was no statistical difference between the DMP group and the OT group (P＞0.05). However, it revealed a significant difference between the DMP group and the GA group (P＜0.01). The calcium content in the DMP group was much lower than that in the GA group. Conclusion: The artery cross-linked by dye-mediated photo-oxidation treatment appears to have less calcification in rabbit allogenic transplantation model.

Key words: Dye-mediated photo-oxidation, Allogenic artery, Transplantation, Anti-calcification