JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro

DAI Li-hua, LI Wei-ning, QIAO Zhi   

  1. Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2007-01-28 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24
  • Contact: QIAO Zhi

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Abstract: Objective: To study the effect of all trans-retinoic acid (ATRA) on the proliferation and apoptosis of human Tenon′s capsule fibroblasts(HTCFs) in vitro. Methods: Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results: Cell cycle distribution of HTCFs showed that cells of the G0/G1 phase increased and of the S phase decreased after the treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose (1×10-9mol/L), and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A “ladder” strand of DNA was produced of the ATRA groups by DNA agarose gel electrophoresis. Conclusion: ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.

Key words: Retinoic acid, Fibroblasts, Tenon′s capsule, Cell proliferation, Apoptosis

CLC Number: 

  • R775.5
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