JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Sequential changes of E-selectin mRNA in human umbilical vein endothelial cells with human cytomegalovirus infection

LIU Zhen-zhen, ZHOU Ya-bin, TANG Wei, LI Shu-ying, WU Jian-min， CHENG Yi-zhe, QI Mei, WANG Hong, LIU Juan, YU Han   

  1. Key Laboratory of Experimental Teratology, Ministry of Education, China; Department of Microbiology, School of Medicine, Shandong University, Jinan 250012, Shandong， China
  • Received:2006-09-29 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24
  • Contact: ZHOU Ya-bin

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Abstract: Objective： By investigating sequential changes of E-selectin in human umbilical vein endothelial cells (HUVEC) by human cytomegalovirus (HCMV) infection, to study the potential mechanism of atherosclerosis（AS）induced by cytomegalovirus infection. Methods： HUVEC was cultured and then infected by HCMV. Expression of E-selectin mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results： Four hours after cytomegalovirus infection, the expression of E-selectin increased. As the time of infection went by, the expression of E-selectin gradually decreased. When the cytomegalovirus infection lasted 48 hours, the expression of E-selectin returned to the fundamental level. There were significant differences among the groups at 0, 4, 8, 12, 24 and 48 hours (P＜0.01). Conclusions： The infection with HCMV can enhance the expression of E-selectin mRNA in HUVEC. So we can say that HCMV may induce the inflammatory reaction by upregulating the E-selectin expression on endothelial cells, which facilitates the occurrence and development of AS.

Key words: Human cytomegalovirus, Human umbilical vein endothelial cells, E-selectin

CLC Number: 

  • R373
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