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Quantification of organic anion transporting polypeptide-E gene expression using the fluorogenic quantitative RT-PCR method

CAI Li1， KONG Qing-nuan2， GUO Cheng-hao3   

  1. 1. Department of Pathology, Anhui Medical University, Hefei 230032, Anhui;2. Department of Pathology, Qingdao Municipal Hospital, Qingdao 266011;3. Department of Pathology, School of Medicine, Shandong University, Jinan 250012
  • Received:2006-10-12 Revised:1900-01-01 Online:2007-01-24 Published:2007-01-24
  • Contact: GUO Cheng-hao

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Abstract: Objective: To establish a fluorogenic quantitative RT-PCR method for detecting the expression of OATP-E gene in tumor cells, and to investigate the expression of organic anion transporting polypeptide-E (OATP-E) gene in breast cancer cell lines MCF-7 and MCF-7/ADR. Method: Fluorogenic quantitative RT-PCR (FQ- RT- PCR) was set up to examine the specific expression of OATP-E gene in MCF-7 and MCF-7/ADR cell lines. Results: After 10 duplicate tests with the FQ-RT-PCR method, the average level of the OATP-E mRNA was 5.47×105copies/μg RNA in MCF-7 cell line and 2.82×104copies/μg RNA in MCF-7/ADR cell line, respectively, therefore the former was 19.4 times greater than the latter. Conclusions: Using FQ-RT-PCR, we can get an accurate quantitative result of OATP-E gene expression . Moreover, OATP-E gene expression in the drug resistant breast cancer cell line MCF-7/ADR is at a lower level than that in cell line MCF-7.

Key words: Polymerase chain reaction, Fluorogenic quantitative, OATP-E, Gene expression

CLC Number: 

  • R361.3
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