Abstract: Objective： To study the effect of the angiogenesis inhibitor SU6668 combined with 5-Fu on liver metastasis in human colon cancer． Methods： The human colon cancer cell line， HT-29 cells were injected intrasplenically into BALB/c nude mice to make the diffuse liver metastasis model．Mice were randomly divided into four groups： SU6668 treatment group， 5-Fu treatment group, SU6668+5-Fu treatment group and control group． Animals were sacrificed after 7 weeks， and their livers were processed for histological examination． Liver metastasis rate and tumor foci in liver were counted． Tumor microvessel density(MVD) and vascular endothelial growth factor(VEGF), and base fibroblast growth factor (bFGF) were determined by the immunohistochemistry SABC method with an image analysis system． Results： SU6668 combined with 5-Fu and SU6668 alone displayed a significant inhibitory effect on liver metastasis compared with the control(P＜0.01), and a significant decrease of the expression of VEGF and bFGF compared with the control(P＜0.05)．　The expression of MVD was also inhibited by SU6668 in combination with 5-Fu or SU6668 alone compared with the control(P＜0.05)．　Conclusion： The angiogenesis inhibitor SU6668 in combination with 5-Fu has an additive effect and inhibitory activity against liver metastasis of human colon cancer， therefore， it might be a safe and effective antitumor strategy．

Key words: Neovascularization, pathologic, 5-Fu, Colon neoplasms, Liver metastasis, Mice, inbred BALB C