亚硒酸钠溶液诱导胃上皮肿瘤SGC-7901细胞凋亡的实验研究

Abstract

Abstract: To investigate whether sodium selenite can efficiently induce SGC7901 cells， and to study the relationship between the apoptosis and the cell cycle. Methods： Being treated with sodium selenite under different concentrations and different times， SGC7901 cells were observed the morphologic changes by electron microscope, and the apoptosis and the cell cycle by TUNEL method and flow cytometry. Results： After 12 hours of exposure to the drug, changes like cell shrinkage, nuclear condensation and formation of apoptotic bodies were exhibited. A typical subdiploid peak before G0/G1 phase was observed by flow cytometry. The apoptosis index was 8.4% to 33.4% by TUNLE. Conclusion： Sodium selenite can induce the apoptosis of SGC7901 cells, which is associated with the blockage of cell cycle.

Key words: Sodium selenite, SGC7901 cells, Apoptosis

ZHAO Yun-dong,SUN Li-yuan,SHAO Shi-he,ZHANG Ya-wen. [J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(11): 1121-1124.

References

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