JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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A simple and sensitive method for screening εPL producing strains from soils

ZHANG Chao,ZHANG Dong-rong,HE Wei,DUAN Zuo-ying,MAO Zhong-gui   

  1. Department of Ferment Engineering, School of Biotechnology, Southern Yangtze University,Wuxi 214036, Jiangsu, China
  • Received:2006-05-10 Revised:1900-01-01 Published:2006-11-24
  • Contact: ZHANG Chao

Abstract: To develop a simple and sensitive method for screening εPL producing strains from soils. Methods: Because dye reacted with the secreted basic polymers by electrostatic interaction and formed special zoon, a basic dye, methylene blue, incorporated in the agar plate was used to detect εPL producers, and then Dragendorff reagent was used to detect alkaloid producers. Result: A over producing strain Z18 was obtained. Conclusion: Z18 is a member of Streptomyces albulus.

Key words: Screening, εpolylysine, Streptomyces albulus ε

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