Journal of Shandong University (Health Sciences) ›› 2026, Vol. 64 ›› Issue (8): 8-13.doi: 10.6040/j.issn.1671-7554.0.2025.1461

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Molecular identification and prevalence of Trypanosoma lewisi in rodents in western areas of Jinan City

MA Chuanmin1, CUI Wen1, LIU Hui1, WANG Gang2   

  1. 1. Institute of Microbiology Testing, Jinan Center for Disease Control and Prevention, Jinan 250118, Shandong, China;
    2. Department of Infectious Diseases, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2026-08-14

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Abstract: Objective To investigate the molecular epidemiological characteristics of Trypanosoma in rodents in western areas of Jinan City and provide evidence for assessing the transmission risk and prevention of trypanosomiasis. Methods Rodent organ samples were collected from western areas of Jinan City. Genus-specific primers were used to amplify the 18S small subunit ribosomal RNA gene(18S rDNA)of Trypanosoma via polymerase chain reaction(PCR). Positive amplification products identified by capillary electrophoresis were sequenced. Sequence alignment and genetic variation analysis of 18S rDNA from positive samples were performed. Results In 2020, a total of 100 rodents were captured from western Jinan. PCR amplification of 18S rDNA from mixed organ samples yielded 5 positive samples, with a Trypanosoma positivity rate of 5.00%. Sequencing and analysis of the five positive 18S rDNA sequences revealed that all detected trypanosomes were Trypanosoma lewisi, with the highest sequence identity reaching 100.00%. Conclusion The trypanosomes carried by rodents in the western areas of Jinan City were exclusively Trypanosoma lewisi, which provides basic data for subsequent comparative studies on trypanosomiasis in this region.

Key words: Trypanosoma lewisi, Molecular epidemiology, Nested polymerase chain reaction, Phylogenetic analysis, Zoonotic pathogen

CLC Number: 

  • R382.2
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