Abstract: To observe the lesion of human umbilical vein endothelial cells (HUVECs) induced by different doses of fluoride. Methods: HUVECs were cultured with fluoride in various concentration (120, 240, 360, 480, 600, 720, 840, 960?μmol/L), 24 hours later, the morphologic changes were observed, the cell activity was measured by MTT method, and the MDA, NO and SOD were determined. Results: The cell increased when treated with fluoride of 120?μmol/L to 240?μmol/L, whereas decreased with fluoride of 600?μmol/L to 960?μmol/L, and the cells were injured. The cell activity of the group of 120?μmol/L and 240?μmol/L significantly enhanced compared with the normal control, but that of the group of over 600?μmol/L markedly lowered compared with the normal control. NO and SOD decreased, but MDA increased in the group of over 240?μmol/L. Conclusions: Fluoride of low concentration can promote the cell activity, but that of high concentration can injure the cell, and the higher the concentration is, the heavier the injury is.

Key words: Fluorid, Human umbilical vein endothelial cells, Hyperplasia, Injury