Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (1): 35-42.doi: 10.6040/j.issn.1671-7554.0.2024.0570

• Clinical Research • Previous Articles     Next Articles

Expression of circ_0000144 in breast cancer and its effect on the proliferation, migration and invasion ability of breast cancer cells

ZHANG Jie1, ZHANG Fangfang1, WANG Jingnan2, LI Zeyu1, SONG Ying1, LI Na1   

  1. 1. Department of Pathology, Xinxiang Medical University, Xinxiang 453003, Henan, China;
    2. Sanquan College of Xinxiang Medical University, Xinxiang 453003, Henan, China
  • Published:2025-02-20

PDF (PC)

327

Abstract: Objective To explore the expression of circ_0000144 in breast cancer tissues and its effect on the proliferation, apoptosis, migration and invasion abilities of breast cancer cells. Methods Quantitative real-time polymerase chain reaction(RT-qPCR)experiments were performed to detect the expression level of circ_0000144 in 49 breast cancer tissues and paired paracancerous normal breast tissue specimens and to analyze the relationship between the expression level of circ_0000144 and the clinicopathological characteristics of breast cancer patients. Normal breast epithelial cells MCF-10A were used as the control, and circ_0000144 expression was detected in breast cancer cell lines(T47D, MCF-7, MDA-MB-231)by RT-qPCR. MCF-7 cells were used as the study object, and the transfected si-circ_0000144 group was set up as the experimental group(si-circ_0000144 group), the transfected si-NC group was set as the saline control group(si-NC group), and a blank control group(Control group)was set at the same time. The cells were cultured with conventional medium without any transfection operation. The abilities of cell proliferation, migration and invasion in breast cancer cells were detected by CCK-8, clone formation assay, flow cytometry, scratch assay and Transwell assay, and the protein expressions of CyclinD1, p21, Bax, Bcl-2, E-cadherin and N-cadherin were detected by Western blotting. Results The expression of circ_0000144 in breast cancer tissues was significantly elevated compared with that in normal tissues adjacent to the cancer(P<0.001), and was closely positively associated with TNM stage and lymph node metastasis(P=0.003, P=0.007). The expressions of circ_0000144 were up-regulated in breast cancer cell lines compared to normal breast epithelial cells MCF-10A(P<0.001). Compared with the Control group or si-NC group, the si-Circ_0000144 group showed reduced abilities of proliferate, migrate and invade(all P<0.001), elevated apoptosis rate(both P<0.001), decreased expression levels of CyclinD1, Bcl-2 and N-cadherin proteins(all P<0.001), and increased expression levels of p21, Bax and E-cadherin proteins(all P<0.001). Conclusion circ_0000144 is highly expressed in breast cancer tissues and cell lines. Down-regulation of circ_0000144 can hinder breast cancer cell proliferation, migration and invasion, and promote its apoptosis. circ_0000144 may become a molecular target for breast cancer treatment.

Key words: Breast cancer, circ_0000144, Proliferation, Apoptosis, Migration, Invasion

CLC Number: 

  • R730
[1] Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 dountries[J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[2] 董相君, 李娟, 孔雪, 等. 环状 RNA hsa_circ_0008591 对乳腺癌细胞生物学行为的影响[J]. 山东大学学报(医学版), 2023, 61(2): 78-87. DONG Xiangjun, LI Juan, KONG Xue, et al. Effects of circular RNA hsa_circ_0008591 on tumor biological behavior of breast cancer cells[J]. Journal of Shangdong University(Health Sciences), 2023, 61(2): 78-87.
[3] Wang SY, Fu SQ, Chen Q, et al. Prognostic role of circular RNAs expression in bladder carcinoma: a meta-analysis[J]. Genet Test Mol Biomarkers, 2020, 24(11): 692-700.
[4] Mi LL, Lei LH, Yin XL, et al. Circ_0000144 functions as a miR-623 sponge to enhance gastric cancer progression via up-regulating GPRC5A[J]. Biosci Rep, 2020, 40(8): 1-12.
[5] Ji F, Lang C, Gao P, et al Knockdown of Circ_0000144 suppresses cell proliferation, migration and invasion in gastric cancer via sponging MiR-217[J]. J Microbiol Biotechnol, 2021, 31(6): 784-793.
[6] Yang B, Wang YW, Zhang K. Interactions between circRNA and protein in breast cancer[J]. Gene, 2024, 895: 148019. doi: 10.1016/j.gene.2023.148019.
[7] Kristensen LS, Jakobsen T, Hager H, et al. The emerging roles of circRNAs in cancer and oncology[J]. Nat Rev Clin Oncol, 2022, 19(3): 188-206.
[8] Zhang Y, Luo J, Yang W, et al. CircRNAs in colorectal cancer: potential biomarkers and therapeutic targets[J]. Cell Death Dis, 2023, 14(6): 353. doi: 10.1038/s41419-023-05881-2.
[9] Li J, Zhang Q, Jiang D, et al. CircRNAs in lung cancer- role and clinical application[J]. Cancer Lett, 2022, 544: 215810. doi: 10.1016/j.canlet.2022.215810.
[10] Lu JC, Ma XM, Lin JH, et al. Circ_0020123 increases ZFX expression to facilitate non-small cell lung cancer progression by sponging miR-142-3p[J]. Cancer Manag Res, 2021, 13(5): 1687-1698.
[11] Fan YX, Shi HY, Hu YL, et al. Circ_0000144 facilitates the progression of thyroid cancer via the miR-217/AKT3 pathway[J]. J Gene Med, 2020, 22(12): e3269. doi:10.1002/jgm.3269.
[12] Wei JM, Wang JM, Gao XB, et al. Identification of differentially expressed circRNAs and a novel hsa_circ_0000144 that promote tumor growth in gastric cancer[J]. Cancer Cell Int, 2019, 19: 268. doi:10.1186/s12935-019-0975-y.
[13] Huang WP, Lu YY, Wang F, et al. Downregulation of circular RNA hsa_circ_0000144 inhibits bladder cancer progression via stimulating miR-217 and suppressing RUNX2 expression[J]. Gene, 2018, 678: 337-342. doi:10.1016/j.gene.2018.08.036.
[14] 韩森吉, 李媛, 李振, 等. IL-37 通过调控 LINC01554/miR-223-3p 轴抑制卵巢癌细胞增殖、迁移和侵袭[J]. 中国免疫学杂志, 2022, 38(13): 1607-1613. HAN Senji, LI Yuan, LI Zhen, et al. IL-37 inhibits proliferation, migration and invasion of ovarian cancer cells by regulating LINC01554/miR-223-3p axis[J]. Chinese Journal of Immunology, 2022, 38(13): 1607-1613.
[15] Benard O, Qian X, Liang HZ, et al. p21CIP1 promotes mammary cancer-initiating cells via activation of Wnt/TCF1/CyclinD1 signaling[J]. Mol Cancer Res, 2019, 17(7): 1571-1581.
[16] Dai XL, Guo X, Liu JJ, et al. Circular RNA circGRAMD1B inhibits gastric cancer progression by sponging miR-130a-3p and regulating PTEN and p21 expression[J]. Aging, 2019, 11(21): 9689-9708.
[17] Sun D, Wang G, Xiao C, et al. Hsa_circ_001988 attenuates GC progression in vitro and in vivo via sponging miR-197-3p[J]. J Cell Physiol, 2021, 236(1): 612-624.
[18] Bi JM, Liu HW, Dong W, et al. Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence[J]. Mol Cancer, 2019, 18(1): 133. doi:10.1186/s12943-019-1060-9.
[19] Jia XB, Zhang Q, Xu L, et al. Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway[J]. Biol Res, 2021, 54(1): 7. doi:10.1186/s40659-021-00330-w.
[20] 魏闫若雪, 李梓绮, 刘春铖, 等. 结直肠癌中SP1的瘤内异质性表达及其临床意义[J]. 山东大学学报(医学版), 2024, 62(5): 89-94. WEI Yanruoxue, LI Ziqi, LIU Chuncheng, et al. Heterogeneous expression of SP1 in colorectal cancer and its clinical significance[J]. Journal of Shandong University(Health Sciences), 2024, 62(5): 89-94.
[21] Si AF, Wang LQ, Miao K, et al. MiR-219 regulates liver cancer stem cell expansion via E-cadherin pathway[J]. Cell Cycle, 2019, 18(24): 3550-3561.
[22] Jin JL, Liu H, Jin MS, et al. Silencing of hsa_circ_0101145 reverses the epithelial-mesenchymal transition in hepatocellular carcinoma via regulation of the miR-548c-3p/LAMC2 axis[J]. Aging(Albany NY), 2020, 12(12): 11623-11635.
[23] Pei C, Wang H, Shi C, et al. CircRNA hsa_circ_0013958 may contribute to the development of ovarian cancer by affecting epithelial-mesenchymal transition and apoptotic signaling pathways[J]. J Clin Lab Anal, 2020, 34(7): e23292. doi: 10.1002/jcla.23292.
[24] Chen WL, Jiang L, Wang JS, et al. Circ-0001801 contributes to cell proliferation, migration, invasion and epithelial to mesenchymal transition(EMT)in glioblastoma by regulating miR-628-5p/HMGB3 axis[J]. Eur Rev Med Pharmacol Sci, 2019, 23(24): 10874-10885.
[25] 张艳霞, 闫燕艳, 邓亮, 等. E-钙黏素和N-钙黏素在胃肠道间质瘤中的表达及其临床意义[J]. 山西大同大学学报(自然科学版), 2023, 39(1): 80-83. ZHANG Yanxia, YAN Yanyan, DENG Liang, et al. Expression and clinical significance of E-cadherin and N-cadherin in gastrointestinal stromal tumors[J]. Journal of Shanxi Datong University(Natural Science Edition), 2023, 39(1): 80-83.
[1] WANG Wei, WANG Yi-Feng, ZHANG Ling-Mei, LIN Qiong-Yan, HUANG Ju. umorigenicity and invasion of side population in human ovarian epithelial cancer OVCAR3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 8-11.
[2] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[3] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[4] CHEN Yang, FENG Ying, LU Xiao, ZHENG Rong. Galectin-3 promotes autophagy and polarization of macrophages via the PI3K/Akt/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 14-22.
[5] YIN Yue, MO Zhenfei, WU Peixin, LIU Jinxia, WEI Yuanhui, REN Jiabo, LI Chunsun. Expression of GPX1 in lung cancer cell lines and its influence on the proliferation, migration, invasion abilities and apoptosis in lung adenocarcinoma cell lines [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 65-73.
[6] GU Chunqing, GUO Ruisi, ZHOU Qinqin, LIU Henghui, BA Wanyu, SUN Shiling, WANG Bing, ZHENG Yuling, WU Suhui. Mechanism of Suanzaoren-Yuanzhi drug pair in the treatment of breast cancer related insomnia based on network pharmacology and animal experiments [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 99-108.
[7] HAN Jueming, WANG Hui, WU Qian, ZHENG Huiling, ZHU Lin. B4GALNT4 promotes proliferation, migration and invasion of lung adenocarcinoma cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 23-31.
[8] LIU Baoguo, SONG Xiang, ZHAO Xiaowen, MAO Yali. Application value of serum STAT5B and NKAIN1 mRNA detection in breast cancer [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 68-74.
[9] WANG Xuemei, YANG Hao, SONG Yang, CHENG Shichao, ZHANG Tingting, WANG Yanchun. Causal association of antidiabetic drugs with female malignant tumours: a two-sample Mendelian randomisation study [J]. Journal of Shandong University (Health Sciences), 2025, 63(6): 67-77.
[10] LI Xiang, ZHANG Yi, WANG Xuechun, XU Mengchao, WANG Yuelan. Research progress on oxidative stress in acute lung injury induced by traumatic brain injury [J]. Journal of Shandong University (Health Sciences), 2025, 63(2): 118-124.
[11] LI Guanqiang, SHI Yucheng, ZHU Kehan, HU Bo, HUANG Xianchen, SUN Yuan, LI Duxin, ZHANG Xicheng. Active peptide SK-14 derived from snail mucus promotes fibroblast proliferation and migration [J]. Journal of Shandong University (Health Sciences), 2025, 63(11): 1-7.
[12] YU Zhigang, ZHENG Chao. Current status, challenges and innovative approaches of multidisciplinary treatment for breast cancer [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 1-9.
[13] . Shandong Province expert consensus on multidisciplinary treatment of breast cancer(2024 edition) [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 10-16.
[14] CHENG Yueqi, WANG Fei, YU Lixiang, ZHENG Chao, YU Zhigang. Progress in the study of trastuzumab-induced cardiotoxicity in HER2-positive breast cancer patients [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 17-24.
[15] WANG Min, LI Xiping, TAN Jun, QIU Mei, HOU Zeyu, TIAN Ying, LUO Hongying, FAN Chaowen, QI Ling, YU Qi, XIE Wei. Lentivirus vector-mediated Gag-Caspase-8 induces apoptosis and S-phase arrest in triple-negative breast cancer primary cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 25-34.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd