Journal of Shandong University (Health Sciences) ›› 2024, Vol. 62 ›› Issue (6): 9-16.doi: 10.6040/j.issn.1671-7554.0.2023.1040

• Preclinical Medicine • Previous Articles     Next Articles

Role of microfibril-associated protein 3 in regulating mesenchymal transition of glioma stem cells

GUO Shuhua1,2, FAN Yang3, TIAN Feng1,2, WANG Chuanxin1,2, DU Lutao1,2, LI Peilong1,2, GUO Xing1,4, XU Shuo1,4   

  1. 1. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Department of Neurosurgery, The First Affiliated Hospital of Shandong First Medical University &
    Shandong Provincial Qianfoshan Hospital, Jinan 250014, Shandong, China;
    4. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2024-07-15

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Abstract: Objective To investigate the expression and clinical significance of microfibril-associated protein 3(MFAP3)in glioblastoma(GBM)and its influence on the malignant biological behavior of glioma stem cells(GSCs). Methods TCGA data and CGGA data were used to analyze the expression of MFAP3 in GBM and its correlation with patient prognosis. Western blotting was used to detect the protein expression level of MFAP3 in GSCs. The effects of MFAP3 knockdown and overexpression on the self-renewal ability and phenotype of GSCs were explored. Results MFAP3 was highly expressed in GBM and correlated with poor prognosis. The expression level of MFAP3 in mesenchymal GSCs was higher than that in preneuronal GSCs. After MFAP3 knockdown, the self-renewal ability of GSCs was weakened, and the protein levels of mesenchymal phenotype markers CD44 and YKL40 were decreased, whereas overexpression exhibited the opposite trends. Conclusion MFAP3 plays an important role in regulating the malignant biological progression of GBM and is expected to be a new clinical diagnostic marker and therapeutic intervention target for GBM.

Key words: Microfibril-associated protein 3, Glioma stem cells, Phenotype transition, Self-renewal, Prognosis

CLC Number: 

  • R739.41
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