Abstract: To study the possibility of culture of human bone marrow mesenchymal stem cells by threedimensional scalffold. Method: Bone marrow mesenchymal stem cells were isolated from human bone marrow and cultured and multiplified, then seeded in the porous partially demineralized bone matrix. The growth and adhesion were observed by DiI label and scanning electromicroscope. Result: About (99.1±1)% of the cells adhered to the porous partially demineralized bone matrix; on the 2nd,　4th and 7th day after the DiI labeled cells were seeded on the scaffold, they were observed under confocal laser scanning microscope , the pore was covered by the cells on the 7th day. On the 7th day, the cells covered the surface of the scaffold; after the scaffold was cut into two parts, it can be observed that the pore in the transverse surface was also filled with cells. Conclusion: Human bone marrow mesenchymal stem cells grow well in the threedimensional environment of partially demineralized bone matrix, which is a good method for culture of human bone marrow mesenchymal stem cells in vitro.

Key words: Bone marrow mesenchymal stem cells, Demineralized bone matrix, Tissue