Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (2): 9-13.doi: 10.6040/j.issn.1671-7554.0.2017.952

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Effects of PRPS2 knockdown on the biological property of cervical cancer cells

CHEN Ying, ZHOU Xiaoqing, QI Mei, ZHANG Weifang, LIU Juan, ZHAO Weiming   

  1. Department of Pathogenic Biology, School of Basic Medical Sciences, Cheeloo College of Medical, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-28

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Abstract: Objective To evaluate the role of phosphoribosyl-pyrophosphate synthetase 2(PRPS2)on the proliferation, metastasis, cell cycle and cell apoptosis of cervical cancer cells. Methods The basal expressions of PRPS2 in cervical cancer cells(HeLa cells)and normal epithelial cells were detected with Western blotting. HeLa cells were transfected with PRPS2-specific siRNA(siPRPS2)via Lipofectamine 2000, and then divided into the knockdown group and control group. The knockdown efficiency of PRPS2 was determined with Western blotting. The cell proliferation, migration and invasion were detected with CCK-8, colony formation and Transwell assays. The effect of PRPS2 on cell cycle and apoptosis were examined with flow cytometry. Results PRPS2 was highly expressed in HeLa cells, and siPRPS2 effectively inhibited the expression of PRPS2(inhibition ratio 70%). CCK-8 and colony formation assays indicated that PRPS2 knockdown significantly inhibited the viability(P<0.001)and proliferation(P<0.01)of HeLa cells. Transwell assays showed that PRPS2 knockdown obviously reduced the number of migrated(P<0.05)and invaded(P<0.001)cells through the membrane. PRPS2 knockdown contributed to G2/M arrest(P<0.001)and apoptosis of cervical cancer cells(P=0.012). Conclusion PRPS2 can promote the proliferation, metastasis and cell cycle of cervical cancer cells.

Key words: Cervical cancer, Phosphoribosyl-pyrophosphate synthetase 2, Proliferation, Migration, Invasion

CLC Number: 

  • R737
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