Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (4): 78-83.doi: 10.6040/j.issn.1671-7554.0.2019.1345

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Airway smooth muscle cells regulate IL-33 expression through TGF-β1/Smad3 signaling pathway to participate in asthma

CAI Qiujing, ZHANG Qian, HE Xuejia, SUN Wenli, GUO Aili, ZHANG Nan, ZHU Weiwei   

  1. Department of Pediatrics, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the signaling mechanism of interleukin(IL)-33 expression and secretion in mouse airway smooth muscle cells. Methods To observe the effect of TGF-β1 with different concentrations(0 ng/mL, 1 ng/mL, 10 ng/mL, 100 ng/mL)on the secretion and expression of IL-33 in mouse airway smooth muscle cells. ELISA was used to detect the concentration of IL-3 in cell culture supernatant of each group. Western blotting was used to detect the expression of IL-33 protein. The inhibitory effect of TGF-β1/Smad3 signaling pathway blocker(SIS3)was observed and the cells were divided into blank group, pretreated TGF-β1 group, unpretreated SIS3 group and pretreated SIS3 group. ELISA was used to detect the concentration of IL-33 in the cell culture supernatant, and Western blotting was used to detect the expressions of Smad3, pSmad3 and IL-33 protein in each group. Results ELISA result showed that the cellular IL-33 concentration in the blank group, 1ng/mL TGF-β1 group, 10 ng/mL TGF-β1 group and 100 ng/mL TGF-β1 group were statistically different (F=106.4, P<0.05). Compared with the blank group, the IL-33 concentration of 10 ng/mL TGF-β1 group and 100 ng/mL TGF-β1 group were increased(P<0.008 3). The IL-33 concentration of 10 ng/mL TGF-β1 group was higher than that of 1ng/mL TGF-β1 group and 100 ng/mL TGF-β1 group(P<0.008 3). Western blotting result showed that the IL-33 protein expression in blank group, 1 ng/mL TGF-β1 group, 10 ng/mL TGF-β1 group and 100 ng/mL TGF-β1 group was statistically different (F=1 613.0, P<0.05). There exists statiscical difference between each two groups with the most significant increase of IL-33 expression in 10 ng/mL TGF-β1 group(P<0.008 3). Westerm blotting result showed that the IL-33 concentration in blank group, pretreatment TGF-β1 group, unpretreatment SIS3 group and pretreatment SIS3 group was statistically different (F=166.7, P<0.05). Compared with the blank group, the IL-33 concentration of pretreatment TGF-β1 group, unpretreatment SIS3 group and pretreatment SIS3 group were increased(P<0.05). There were statistical difference of Smad3, pSmad3, IL-33 protein expressions among the blank group, pretreatment TGF-β1 group, unpretreatment SIS3 group and pretreatment SIS3 group［(F=4 752.0,P<0.05),(F=4 330.0,P<0.05),(F=2 791.0,P<0.05)］. Compared with the blank group, the Smad3, pSmad3 and IL-33 protein expressions were increased in pretreatment TGF-β1 group and pretreatment SIS3 group while those decreased in unpretreatment SIS3 group(P<0.05). Conclusion TGF- β1 with a certain concentration can stimulate the expression and secretion of IL-33 in mouse airway smooth muscle cells, and TGF-β1/Smad3 signaling pathway can regulate this process involved in the asthma mechanism.

Key words: Transforming growth factor-β1/Smad3 signal pathway, Interluekin-33, Mouse airway smooth muscle cell, Asthma

CLC Number: 

  • R574
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