Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (2): 80-87.doi: 10.6040/j.issn.1671-7554.0.2018.1165

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Effect of FK506 binding protein 52 on proliferation of human endometrial stromal cells

YAN Huili1, WEI Muyun2, YAN Lei3, ZHAO Yueran2,3   

  1. 1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China;
    2. Department of Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Center for Reproductive Medicine, Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the effects of FK506 binding protein 52(FKBP52)on proliferation of human endometrial stromal cells(HESCs), and provide the new basis and target for treatment of thin endometrium. Methods Normal HESCs were isolated and cultured. After infecting HESCs with lentiviruses contain GFP-FKBP52 or transfecting HESCs with FKBP52-targeting small interfering RNA(siRNA)to overexpress or knockdown FKBP52 respectively, the cells proliferation and colony-formation were compared with CCK8 assay and plate clone formation assay. Besides, changes in cell cycle were detected by flowcytometry and expression levels of FKBP52 and cell cycle-related proteins were examined by Western blotting. Results After overexpressing FKBP52, the proliferation activity of HESCs was enhanced(P<0.05), and the clone formation ability of cells was improved(P<0.01). The process of cell cycle was accelerated(P<0.01)and the expression levels of cell cycle dependent kinase 4(CDK4), CyclinD1, cell cycle dependent kinase 2(CDK2)and CyclinE1 were increased(P<0.05). Affected by FKBP52 knockdown in transfected cell, the proliferation capacity of endometrial stromal cells was reduced(P<0.05), and the clone formation capacity was weakened(P<0.05). The cell cycle process was blocked(P<0.05)and expression levels of CDK4, CyclinD1,CDK2 and CyclinE1 were suppressed(P<0.05). Conclusion FKBP52 can control the proliferation of HESCs by regulating the cell 山 东 大 学 学 报 (医 学 版)57卷2期 -阎慧丽,等.FK506结合蛋白52对人子宫内膜间质细胞增殖作用的影响 \=- cycle and can be considered as a target for the treatment of thin endometrium.

Key words: FK506 binding protein 52, Cell proliferation, Cell cycle, Thin endometrium, Endometrial stromal cell

CLC Number: 

  • R711.7
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