JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

WANG Dong-hai,LI Xin-gang,QU Xun,YANG Mei-xiang,BAO Xiu-feng,LI Gang,GONG Song-feng   

  • Received:2006-03-29 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24
  • Contact: WANG Dong-hai

Abstract: To study the effective method of isolation、culture and proliferation of rat bone marrowderived dendritic cells(DC) in vitro. Methods: Nonadherent bone marrow monocytes were harvested by centrifugation and cultured in RPMI1640 with cytokines( rGMCSF and rIL4,5?ng/ml). Morphology, expression of special surface marker and functional ability were observed. Results: Morphomogical property of typical DC was observed in DC progenitors at day 10 of culture. Positive special surface marker of OX62 was observed with immunoflourense, and about 86% OX62+ cells was found by Flow cytometry analysis. To syngeneic spleen lymphocytes, the strongest proliferation of MLR induced by DC at day 10 of culture. MLR of syngeneic spleen lymphocytes was stronger than that of heterogeneous spleen lymphocytes (P<0.05). Conclusion: Improved Chen－Woan method is favorable in inducing and proliferating rat bone marrowderived DC in vitro.

Key words: Dendritic cell, Bone marrow, Cytokine, Rat, Wistar

[1] YU De-Xin, CAEI Ti-Gong, MA Xiang-Xing, ZHANG Xiao-Ming, LI Chuan-Fu. Angiogenesis and maturation of hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 51-54.
[2] WUYi-Na, GE Zhi-Ming, LI Fang, HE Hong, JIANG Hong, ZHANG Yun. Expressions of angiotensin converting enzyme 2 mRNA and protein in the kidneys in spontaneously hypertensive rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 5-.
[3] ZHU Lin, HU San-Yuan, ZHANG Guang-Yong, DING Xiang-Jiu. Protection of PGE2 against intestinal mucosa injuries in obstructed jaundice rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 12-.
[4] SUN Tao, ZHANG Dao-Lai, XIE Shan-Shan, WANG Yu-Zhuo, FENG Yu-Xin, XIN Hua. Effect of ethanol on expression of connexin 43 in primary culture of neural precursor cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 20-.
[5] ZHANG Dao-Lai, SUN Tao, XIE Shan-Shan, WANG Yu-Zhuo, ZHAO Ling, FENG Yu-Xin, XIN Hua. Developmental expression of NMDAR1 subunit on cultured cerebral cortical neurons of fetal rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 28-32.
[6] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[7] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[8] ZHANG Xiao-Yan, LI Gang, WANG Ling-Beng. Effects of different dosages of Sufentanil in induced general anesthesia  period on blood rheology cortisol in spinal operations [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 76-79.
[9] WANG Xin, XING Chun-Yan, YANG Yan-Ping. Serum phosphopyruvate hydratase in diagnosis of invasive Candida albicans infection [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 92-94.
[10] DONG Ping, SHEN Haitao, QIAO Yaqin, LU Yan. Role of autophagy in the regulation of APAP liver injury and liver regeneration process [J]. Journal of Shandong University (Health Sciences), 2026, 64(5): 42-49.
[11] DONG Zihao, LIU Zhe, WANG Hao, YU Chenxi, YANG Guang, FENG Haotian. Incidence of early postoperative complications following different capsulotomy methods in hip arthroscopy [J]. Journal of Shandong University (Health Sciences), 2026, 64(5): 83-87.
[12] CHEN Yumeng, ZHANG Yue, ZHANG Wulin, YANG Guoxing, XU Yanhui, HAN Aijun, LIU Caijuan, GUO Yuyu, CHEN Zhimin. Evaluating the efficacy of large language models in answering questions from parents of children with congenital lens dislocation [J]. Journal of Shandong University (Health Sciences), 2026, 64(5): 88-95.
[13] MA Yihan, YUAN Xinyun, LI Jiani, HAN Xu, DIAO Qidi, WANG Yan, CHU Xu, ZHANG Kun, HU Lei. Relationship between childhood abuse and frequent nightmares in adults with depressive disorders: mediating effect of somatic symptoms and moderating role of social support [J]. Journal of Shandong University (Health Sciences), 2026, 64(5): 96-105.
[14] WANG Ye, WANG Gang, NAN Haiou, CAI Yuling, LI Jiayu, WANG Haifeng, YAN Yingjie, JIANG Zhiwei. Correlation between perioperative heart rate variability and inflammatory indicators in gastric cancer patients [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 23-30.
[15] LI Jingxiu, LI Zhonghui, LI Wentian, WANG Yunan, SONG Zehui, ZHUANG Dayong, HE Qingqing, ZHOU Peng. Preliminary application of photoelectric detection devices in thyroid cancer surgery [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 31-37.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd